Description

p-Nitrophenyl Phosphate Magnesium Salt is a high-purity chromogenic substrate widely used in enzymatic assays. This compound is critical for the detection and quantification of alkaline phosphatase activity, a key enzyme in diagnostic and research applications. It is an essential reagent for clinical diagnostics, life science research, and biopharmaceutical quality control, where reliable and sensitive detection is paramount.

Application

• Clinical Diagnostics: Primary substrate in colorimetric assays for measuring alkaline phosphatase (ALP) levels in serum, a vital marker for liver and bone disorders.
• Enzyme-Linked Immunosorbent Assay (ELISA): Used as a detection reagent in ELISA kits for various antigens and antibodies, providing a colorimetric readout.
• Molecular Biology Research: Essential for non-radioactive detection in techniques like Western blotting and nucleic acid hybridization (e.g., using alkaline phosphatase-conjugated probes).
• Pharmaceutical Quality Control: Employed in potency testing and impurity profiling of biopharmaceuticals that utilize alkaline phosphatase in their manufacturing or testing processes.
• Food Safety Testing: Used in enzymatic methods to detect certain contaminants or to verify pasteurization efficacy.
• In Vitro Diagnostics (IVD) Manufacturing: A key raw material in the formulation of diagnostic reagents and automated chemistry analyzer kits.

Basic Information

Product Name	p-Nitrophenyl Phosphate Magnesium Salt
CAS No.	32348-90-6
Molecular Formula	C6H4MgNO6P (commonly represented as the hexahydrate, C6H4MgNO6P·6H2O)
Molecular Weight	Anhydrous: 233.37 g/mol; Hexahydrate: 341.46 g/mol
Synonyms	PNPP Magnesium Salt; 4-Nitrophenyl Phosphate Magnesium Salt; pNPP-Mg; Phosphoric Acid 4-Nitrophenyl Ester Magnesium Salt; Magnesium 4-Nitrophenyl Phosphate; ALP Substrate; Alkaline Phosphatase Substrate; Diagnostic Grade PNPP; Disodium p-nitrophenyl phosphate (related acid form)
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl Phosphate Magnesium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is tested to meet stringent specifications for identity, purity, and functional activity. A comprehensive Certificate of Analysis (COA) detailing assay, impurity profile, and solubility is provided with every shipment, supporting compliance in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Solubility (in water)	Clear, colorless solution at 10 mg/mL
Absorbance (at 405 nm)	Meets functional activity criteria
Heavy Metals (as Pb)	≤ 20 ppm
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.