Description

n-[[[3-Carboxy-4-(6-Hydroxy-3-Oxo-3H-Xanthen-9-Yl)Phenyl]Amino]Thioxomethyl]-L-Trp-L-Met-L-Asp-Phe-Nh2 is a sophisticated synthetic peptide derivative incorporating a fluorescein-like xanthene moiety, designed for advanced biochemical research and diagnostic applications. This compound is engineered to provide a highly specific and sensitive probe, leveraging its intrinsic fluorescence and peptide-based targeting capabilities. It is primarily utilized by researchers and developers in the fields of bioconjugation, assay development, and molecular imaging, where precise labeling and detection are paramount.

Application

• Fluorescent Labeling Agent: For covalent conjugation to proteins, antibodies, or other biomolecules to create fluorescent probes for immunoassays (e.g., ELISA, Western Blot) and flow cytometry.
• Protease Activity Assays: Serves as a fluorogenic substrate in enzyme activity studies, particularly for proteases that cleave the specific peptide sequence (Trp-Met-Asp-Phe).
• Molecular Imaging Probes: Used in the development of targeted imaging agents for cellular and in vitro diagnostic applications.
• Bioconjugation Research: Acts as a model compound in developing new conjugation chemistries and linker technologies due to its reactive thioxomethyl group and fluorescent reporter.
• Diagnostic Kit Development: A key component in the formulation of high-sensitivity diagnostic kits for clinical and research laboratories.
• Cell Biology Studies: For tracking and visualizing specific cellular processes or protein localization when conjugated to targeting ligands.

Basic Information

Product Name	n-[[[3-Carboxy-4-(6-Hydroxy-3-Oxo-3H-Xanthen-9-Yl)Phenyl]Amino]Thioxomethyl]-L-Trp-L-Met-L-Asp-Phe-Nh2
CAS No.	31966-60-6
Molecular Formula	C58H55N9O11S2
Molecular Weight	1118.23 g/mol
Synonyms	Fluorescein-Peptide Conjugate; Xanthene-Peptide Hybrid; F-Peptide (Trp-Met-Asp-Phe-NH2); Thioxomethyl Fluorescein Peptide; Fluorescent Peptide Substrate; 5(6)-Carboxyfluorescein-Trp-Met-Asp-Phe-amide; Protease Substrate with Fluorescein; CAS 31966-60-6
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical is governed by a stringent quality management system. Every batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation, mass spectrometry (MS) for molecular weight verification, and absorbance/fluorescence spectroscopy to ensure optimal performance characteristics. Certificates of Analysis (COA) detailing all specifications and test results are provided to guarantee traceability, consistency, and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Peptide Content	≥ 80.0% (by weight)
Water Content (KF)	≤ 5.0%
Absorbance Maximum	490 - 495 nm
Emission Maximum	515 - 520 nm
Solubility	Soluble in DMSO, DMF; slightly soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.