Description

5-Iodo-2'-Deoxycytidine 5'-Triphosphate Sodium is a modified nucleotide triphosphate (dNTP) analog, a critical reagent for advanced molecular biology and diagnostic applications. Its core value lies in its ability to incorporate iodine atoms into nucleic acid chains, enabling precise labeling and detection. This compound is essential for researchers and manufacturers in molecular diagnostics, next-generation sequencing (NGS), and biopharmaceutical development who require high-purity, reliable building blocks for nucleic acid synthesis.

Application

• Next-Generation Sequencing (NGS): Used as a substrate for DNA polymerases in sequencing-by-synthesis platforms, particularly useful for specialized assays.
• Polymerase Chain Reaction (PCR): Employed in specialized PCR applications, including the synthesis of iodine-labeled DNA probes for enhanced detection.
• DNA Labeling & Probe Synthesis: Acts as a direct precursor for creating non-radioactive, iodine-tagged nucleic acid probes used in hybridization assays like FISH (Fluorescence In Situ Hybridization) and microarrays.
• Enzymatic DNA Synthesis: Serves as a modified nucleotide building block in template-independent enzymatic synthesis methods for producing oligonucleotides with specific modifications.
• Structural Biology Studies: Utilized in X-ray crystallography and other structural analysis techniques where heavy atom incorporation (iodine) is required for phasing.
• Antiviral & Anticancer Research: Investigated as a potential chain terminator or substrate analog in the study of viral polymerases and for developing novel therapeutic agents.

Basic Information

Product Name	5-Iodo-2'-Deoxycytidine 5'-Triphosphate Sodium
CAS No.	31747-59-8
Molecular Formula	C9H13IN3NaO13P3 • xH2O
Molecular Weight	Approx. 675.0 (Anhydrous) - Contact for details on specific hydrate.
Synonyms	5-Iodo-dCTP Sodium Salt; 5-Iodo-2'-deoxycytidine-5'-triphosphate trisodium salt; dCTP-I; Iododeoxycytidine triphosphate; 2'-Deoxy-5-iodocytidine-5'-triphosphate sodium; 5-Iodo-2'-deoxycytidine 5'-(tetrahydrogen triphosphate) trisodium salt; 5-I-dCTP; Sodium 5-iodo-2'-deoxycytidine-5'-triphosphate
EINECS	Contact for details.

Quality Control

Our 5-Iodo-2'-Deoxycytidine 5'-Triphosphate Sodium is manufactured under strict quality systems to ensure batch-to-batch consistency and suitability for sensitive life science applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity, NMR for structural confirmation, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles (including related nucleotides and residual solvents) is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (as sodium salt)
Water Content (KF)	≤ 5.0%
pH (1% Solution)	6.0 - 8.0
Endotoxin (LAL)	< 10 EU/mg
Filter Sterility	Pass (0.2 µm filter)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.