Description

4-Thiouridine Triphosphate is a chemically modified nucleotide triphosphate analog, distinguished by the presence of a thiol group on the uracil base. This unique modification makes it a critical reagent for specialized biochemical research and diagnostic applications, particularly in nucleic acid labeling and crosslinking studies. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biotechnology companies engaged in advanced molecular biology and genomics.

Application

• Nucleic Acid Labeling and Detection: Acts as a substrate for in vitro transcription to produce RNA probes containing thiol groups for subsequent conjugation with fluorescent or affinity tags.
• Crosslinking Studies: Used in photo-crosslinking experiments (e.g., 365 nm UV light) to study RNA-protein or RNA-RNA interactions due to its photoreactive thione group.
• Structural Biology: Facilitates the site-specific incorporation of heavy atoms or biophysical probes into RNA for analysis by techniques like X-ray crystallography or NMR spectroscopy.
• SELEX and Aptamer Development: Employed in the selection of thio-modified aptamers (thioaptamers) which often exhibit enhanced nuclease resistance and binding affinity.
• Diagnostic Assay Development: Serves as a key component in the synthesis of modified nucleotides for advanced molecular diagnostic tests, including next-generation sequencing platforms.
• Enzyme Mechanism Studies: Used as a substrate analog to investigate the specificity and catalytic mechanisms of RNA polymerases and other nucleotide-processing enzymes.

Basic Information

Product Name	4-Thiouridine Triphosphate
CAS No.	31556-28-2
Molecular Formula	C9H15N2O14P3S
Molecular Weight	516.19 g/mol
Synonyms	4-Thio-UTP; 4sUTP; 4-Thiouridine 5'-triphosphate; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-4-thioxo-1,2,3,4-tetrahydropyrimidin-2-one 5'-(trihydrogen triphosphate); Uridine 5'-(tetrahydrogen triphosphate), 4-thio-; 4-Thiouridine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our 4-Thiouridine Triphosphate is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment and identity confirmation via UV-Vis spectroscopy, to ensure it meets the high standards required for sensitive research applications. A Certificate of Analysis (COA) detailing purity, identity, and stability is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation and storage under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (UV-Vis)	Characteristic absorption spectrum with λmax ~330-340 nm
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
Biological Activity	Suitable for in vitro transcription

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.