Description

Disuccinimidyl Succinate is a homobifunctional N-hydroxysuccinimide (NHS) ester crosslinker widely used in bioconjugation chemistry. This compound is essential for creating stable amide bonds between primary amines, enabling the precise modification and labeling of proteins, peptides, and other biomolecules. It is a critical reagent for researchers and manufacturers in pharmaceutical development, proteomics, diagnostic assay production, and antibody-drug conjugate (ADC) synthesis.

Application

• Protein-Protein and Protein-Peptide Crosslinking: For studying protein interactions, complex formation, and creating immunogens.
• Antibody-Drug Conjugate (ADC) Linker Chemistry: As a key component in the synthesis of stable, cleavable linkers for targeted cancer therapies.
• Biomolecule Immobilization: Covalent attachment of proteins, antibodies, or enzymes to solid supports (e.g., beads, plates, biosensors) for assays and purification.
• Fluorescent and Affinity Labeling: Conjugation of dyes, biotin, or other tags to primary amine-containing molecules for detection and isolation.
• Polymer and Material Science: Modification of amine-functionalized polymers, dendrimers, or nanoparticles for biomedical applications.
• Diagnostic Reagent Manufacturing: Production of enzyme-linked immunosorbent assay (ELISA) components and other diagnostic kits.

Basic Information

Product Name	Disuccinimidyl Succinate
CAS No.	30364-60-4
Molecular Formula	C12H10N2O8
Molecular Weight	310.22 g/mol
Synonyms	DSS; Succinic acid bis(2,5-dioxopyrrolidin-1-yl) ester; Bis(N-succinimidyl) succinate; N,N'-Disuccinimidyl succinate; 1,1'-(Butanedioyl)bis(1H-pyrrole-2,5-dione); BS3 (commonly associated with the water-soluble sulfonated analog); Disuccinimidyl butanedioate
EINECS	250-149-5

Quality Control

Our Disuccinimidyl Succinate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMF, DMSO, acetonitrile; insoluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.