Description

Hydrocortisone-(1,2-3H(N)) is a high-specific-activity, tritium-labeled derivative of the endogenous glucocorticoid hormone hydrocortisone (cortisol). This radiochemical is essential for advanced research requiring precise tracking and quantification of steroid hormone pathways. It serves as a critical tool for scientists in pharmaceutical development, endocrinology, and molecular biology. The product is supplied with a defined specific activity and radiochemical purity to ensure reliable and reproducible experimental results.

Application

• Receptor Binding Assays: Used as a high-affinity radioligand for studying glucocorticoid receptor (GR) binding kinetics, affinity, and density in competitive binding studies.
• Metabolic Pathway Tracing: Enables the detailed investigation of hydrocortisone metabolism, biotransformation, and catabolic pathways in in vitro and ex vivo systems.
• Drug Discovery & Development: Facilitates high-throughput screening (HTS) and mechanistic studies for novel anti-inflammatory agents and glucocorticoid receptor modulators.
• Absorption, Distribution, Metabolism, and Excretion (ADME) Studies: A vital tracer for pharmacokinetic research, helping to elucidate the distribution and clearance of corticosteroid drugs.
• Endocrinology Research: Applied in studies of hypothalamic-pituitary-adrenal (HPA) axis regulation, stress response, and steroid hormone action at the cellular level.
• Autoradiography: Used for the spatial localization and visualization of glucocorticoid receptor sites in tissue sections.

Basic Information

Product Name	Hydrocortisone-(1,2-3H(N))
CAS No.	3844-64-2
Molecular Formula	C21H283H2O5
Molecular Weight	Contact for details
Synonyms	[1,2-3H]Cortisol; Tritiated Cortisol; 11β,17α,21-Trihydroxy-4-pregnene-3,20-dione-1,2-3H(N); Cortisol-1,2-3H; 4-Pregnene-11β,17α,21-triol-3,20-dione-1,2-3H; Radiolabeled Hydrocortisone; [1,2-3H]Hydrocortisone
EINECS	223-332-1

Quality Control

Every batch of Hydrocortisone-(1,2-3H(N)) is manufactured and analyzed under strict quality systems. The product undergoes comprehensive testing to ensure high radiochemical purity and specific activity as specified. Analytical methods include HPLC with radiometric detection and TLC. A detailed Certificate of Analysis (COA) is provided with each shipment, containing batch-specific data on purity, specific activity, concentration, and solvent information.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. For long-term storage, keep at -70°C to minimize radiolytic decomposition. The product is strictly light-sensitive and must be protected from all light exposure. Store away from strong acids and organic solvents.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	30-60 Ci/mmol (1.11-2.22 TBq/mmol)
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 95%
Concentration	1 mCi/mL (37 MBq/mL)
Solvent	Ethanol:Water (9:1)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.