Description

5-Fluorouridine 5'-Triphosphate is a fluorinated nucleotide analog essential for biochemical research and pharmaceutical development. This compound serves as a critical building block for studying RNA metabolism, enzyme mechanisms, and as a potential antiviral or anticancer agent. It is primarily utilized by researchers and manufacturers in the fields of molecular biology, biochemistry, and medicinal chemistry for applications ranging from enzymatic synthesis to drug discovery.

Application

• Enzymatic RNA Synthesis: Acts as a substrate for RNA polymerases (e.g., T7 RNA polymerase) to incorporate 5-fluorouridine into RNA strands for structural and functional studies.
• Biochemical Assay Development: Used in high-throughput screening assays to identify and characterize inhibitors of viral RNA-dependent RNA polymerases.
• Mechanistic Studies: Employed to investigate the kinetics and fidelity of nucleic acid polymerases and other nucleotide-processing enzymes.
• Antiviral Research: Serves as a precursor for the investigation of nucleoside analog drugs targeting viral replication, particularly for RNA viruses.
• Medicinal Chemistry: A key intermediate in the research and development of novel fluorinated nucleoside-based therapeutics.
• Metabolic Pathway Analysis: Used as a tracer or probe to study nucleotide salvage pathways and RNA turnover in cellular systems.

Basic Information

Product Name	5-Fluorouridine 5'-Triphosphate
CAS No.	3828-96-4
Molecular Formula	C9H13FN3O14P3
Molecular Weight	523.11 g/mol
Synonyms	5-Fluoro-UTP; 5-Fluorouridine triphosphate; 5-Fluoro-uridine-5'-triphosphate; 5-Fluorouridine 5'-(tetrahydrogen triphosphate); 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(6-hydroxy-5-fluoro-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl) 1-(trihydrogen diphosphate) methyl trihydrogen diphosphate; 5-F-dUTP; F-UTP
EINECS	Contact for details

Quality Control

Our 5-Fluorouridine 5'-Triphosphate is manufactured under stringent quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards required for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 3.0%
pH (1% Solution)	6.0 - 8.0
Endotoxin	< 10 EU/mg (if applicable)
Biological Activity	Functional in enzymatic assay (per COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.