Description

4-Nitrophenyl-α-D-Glucopyranoside is a chromogenic substrate widely used in enzymatic assays. This compound is critical for the detection and quantification of α-glucosidase activity, serving as a key reagent in biochemical research and diagnostic applications. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics industries.

Application

• Enzymatic Substrate for α-Glucosidase: Primary chromogenic substrate for the spectrophotometric determination of α-glucosidase enzyme activity in research and diagnostic kits.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and characterization of glycoside hydrolases.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic assays for metabolic disorders, such as Pompe disease and diabetes-related enzyme panels.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) campaigns to identify potential therapeutic agents targeting carbohydrate metabolism.
• Microbiology: Used in specific tests for bacterial identification based on glycosidase activity patterns.
• Quality Control in Enzyme Production: Serves as a standard substrate for assessing the purity and specific activity of commercial α-glucosidase preparations.

Basic Information

Product Name	4-Nitrophenyl-α-D-Glucopyranoside
CAS No.	3767-28-0
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	p-Nitrophenyl-α-D-glucopyranoside; α-PNPG; 4-Nitrophenyl α-D-glucoside; p-Nitrophenyl α-D-glucopyranoside; PNPG; α-4-Nitrophenylglucopyranoside; 1-O-(4-Nitrophenyl)-α-D-glucopyranose; 4-Nitrophenyl-α-D-glucoside
EINECS	223-197-8

Quality Control

Our 4-Nitrophenyl-α-D-Glucopyranoside is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.