Description

Thymidine 5'-Triphosphate Sodium Salt is a high-purity nucleotide essential for DNA synthesis and molecular biology research. Its primary value lies in providing a reliable and consistent source of dTTP, a critical building block for enzymatic reactions like PCR, DNA sequencing, and cDNA synthesis. This product is indispensable for biotechnology companies, pharmaceutical R&D laboratories, and academic institutions engaged in genomics, diagnostics, and life science studies.

Application

• Polymerase Chain Reaction (PCR): As one of the four essential deoxynucleoside triphosphates (dNTPs) for DNA amplification.
• DNA Sequencing: Used in Sanger (dideoxy) sequencing and next-generation sequencing (NGS) platforms.
• cDNA Synthesis: A key component in reverse transcription reactions for generating complementary DNA from RNA templates.
• Site-Directed Mutagenesis: Employed in enzymatic methods for introducing specific mutations into DNA sequences.
• DNA Labeling: Can be used in reactions incorporating radioactive or fluorescent tags for detection and analysis.
• Enzymatic DNA Synthesis: Serves as a substrate for terminal deoxynucleotidyl transferase (TdT) and other DNA polymerases.
• Biochemical Research: Used in studies of enzyme kinetics, DNA repair mechanisms, and nucleic acid metabolism.

Basic Information

Product Name	Thymidine 5'-Triphosphate Sodium Salt
CAS No.	3624-46-2
Molecular Formula	C10H14N2Na2O14P3 (typically hydrated)
Molecular Weight	Approx. 564.1 g/mol (anhydrous basis)
Synonyms	dTTP Sodium Salt; 2'-Deoxythymidine 5'-triphosphate sodium salt; Thymidine triphosphate sodium salt; dTTP-Na2; 5'-TTP Sodium Salt; TTP; Deoxythymidine triphosphate sodium salt; Thymidine 5'-(tetrahydrogen triphosphate) disodium salt
EINECS	222-831-3

Quality Control

Our Thymidine 5'-Triphosphate Sodium Salt is manufactured under strict quality systems to ensure it meets the exacting standards required for sensitive molecular biology applications. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing purity, endotoxin levels, and absence of contaminating nucleases is provided with every shipment to guarantee batch-to-batch consistency and performance reliability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Aliquot into single-use vials to minimize freeze-thaw cycles and maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95%
pH (Solution)	6.5 - 7.5
Water Content (KF)	≤ 5.0%
Endotoxin	< 0.1 EU/μmol
Nuclease Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.