Description

Dl-Dithiothreitol CAS NO 3483-12-7 is a versatile and powerful reducing agent, widely recognized for its ability to cleave disulfide bonds in proteins and peptides. This property is fundamental for maintaining the biological activity of sensitive biomolecules in research and manufacturing. It is an essential reagent for biochemists, molecular biologists, and pharmaceutical scientists working in protein chemistry, nucleic acid research, and diagnostic assay development.

Application

• Protein Biochemistry: Reduction and denaturation of proteins for SDS-PAGE, Western blotting, and structural analysis.
• Molecular Biology: Protection of sulfhydryl groups in enzymes (e.g., reverse transcriptase) and prevention of disulfide bond formation during nucleic acid manipulation.
• Pharmaceutical Development: Used in the formulation and stabilization of therapeutic proteins and peptides.
• Diagnostic Reagents: A key component in buffer systems for immunoassays and clinical diagnostic kits to maintain reagent stability.
• Cell Culture & Biotechnology: Addition to media to reduce oxidative stress and improve cell viability in certain culture conditions.
• Chemical Synthesis: Acts as a selective reducing agent in organic synthesis for the protection and deprotection of thiol groups.

Basic Information

Product Name	Dl-Dithiothreitol
CAS No.	3483-12-7
Molecular Formula	C4H10O2S2
Molecular Weight	154.25 g/mol
Synonyms	DTT; DL-Dithiothreitol; (±)-1,4-Dithiothreitol; Cleland's Reagent; Dithioerythritol; 2,3-Dihydroxy-1,4-butanedithiol; (2S,3S)-1,4-Dimercapto-2,3-butanediol (racemic); Threo-1,4-dimercapto-2,3-butanediol
EINECS	222-468-4

Quality Control

Our Dl-Dithiothreitol is manufactured under strict quality management systems. Each batch is tested to ensure high purity and consistency, meeting the rigorous demands of research and industrial applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) for long-term stability. This product is hygroscopic (moisture-sensitive) and easily oxidized; minimize exposure to air and moisture during handling. For extended storage under an inert atmosphere, please contact us for specific recommendations.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 99.0%
Melting Point	40 - 44 °C
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Optical Rotation	± 0.5° (c=1 in water)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.