Description

4-Nitrophenyl-n-Acetyl-β-D-Glucosaminide is a specialized chromogenic substrate widely used in enzymatic assays. This compound matters for its high sensitivity and specificity in detecting and quantifying the activity of N-acetyl-β-D-glucosaminidase (NAGase) and related enzymes. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for enzyme kinetics studies and diagnostic test development.

Application

• Enzyme Activity Assays: Primary substrate for the colorimetric determination of N-acetyl-β-D-glucosaminidase (NAGase) activity in research and clinical settings.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for detecting lysosomal storage disorders and renal tubular damage.
• Biochemical Research: Used in studies of carbohydrate-active enzymes, glycosidase inhibition, and metabolic pathway analysis.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) for identifying potential glycosidase inhibitors as drug candidates.
• Microbiology: Applied in assays to study bacterial and fungal glycosidases for microbiological identification and research.
• Quality Control (QC): Serves as a reference standard in QC laboratories for calibrating enzymatic diagnostic methods.

Basic Information

Product Name	4-Nitrophenyl-n-Acetyl-β-D-Glucosaminide
CAS No.	3459-18-5
Molecular Formula	C14H18N2O8
Molecular Weight	342.30 g/mol
Synonyms	4-Nitrophenyl N-acetyl-β-D-glucosaminide; PNP-NAG; p-Nitrophenyl N-acetyl-β-D-glucosaminide; NAG-4NP; 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside; 2-Acetamido-2-deoxy-4-nitrophenyl-β-D-glucopyranoside; β-4-Nitrophenyl N-acetylglucosaminide; N-Acetyl-β-D-glucosaminide 4-nitrophenyl
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-n-Acetyl-β-D-Glucosaminide is produced under a rigorous quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, assay, and related substance profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.