Description

5-Bromouridine Triphosphate is a halogenated ribonucleoside triphosphate analog widely used as a biochemical tool for RNA labeling, sequencing, and structural studies. It serves as a critical substrate for T7 RNA polymerase and other viral or cellular RNA-dependent RNA polymerases, enabling site-specific incorporation of bromine atoms into nascent RNA strands for downstream detection and analysis. Researchers in molecular biology, virology, and structural genomics rely on this high-purity reagent for applications including X-ray crystallography phasing, cryo-EM sample preparation, and photo-crosslinking studies. The compound is commercially supplied as the sodium salt and is referenced globally as 5-Bromouridine Triphosphate CAS NO 3398-50-3.

Application

• Site-specific radiolabel-free RNA labeling for gel electrophoresis and autoradiography alternatives
• Phasing agent in X-ray crystallography of RNA-protein complexes
• Substrate for in vitro transcription assays with T7, SP6, and mutant RNA polymerases
• Photo-crosslinking probe for mapping RNA–protein interaction interfaces
• Building block in synthetic biology workflows for engineered ribozymes and aptamers
• Tool compound in antiviral drug discovery targeting RNA-dependent RNA polymerases (e.g., SARS-CoV-2 nsp12)
• Mass spectrometry standards for nucleotide metabolism profiling
• Component in diagnostic assay development for pathogen RNA detection

Basic Information

Product Name	5-Bromouridine Triphosphate
CAS No.	3398-50-3
Molecular Formula	C9H14BrN4O13P3
Molecular Weight	568.04 g/mol
Synonyms	5-Br-UTP; 5-Bromouridine 5'-triphosphate; 5-Bromouridylic acid triphosphate; Bromouridine triphosphate; 5-Bromouridine-5'-triphosphoric acid; 5-Bromo-UTP; 5-Bromouridine-5'-O-(tetrahydrogen triphosphate); 5-Bromouridine triphosphate sodium salt; 5-Bromouridine-5'-triphosphate trisodium salt
EINECS	222-255-1

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for identity (IR, HPLC retention time), purity (≥98.5% by HPLC), and absence of enzymatic contaminants (nuclease-, phosphatase-, and protease-free). Testing conforms to ACS Reagent Grade specifications for nucleotide triphosphates, with additional validation for RNA polymerase compatibility and low endotoxin levels (≤0.1 EU/μg).

Storage

Preserve in a tightly closed container, protected from light. Store at –20 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, minimize exposure to ambient humidity during handling. Allow vial to equilibrate to room temperature in a desiccated environment before opening.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.5%
Related Substances (HPLC)	≤1.5% total impurities
Uridine Triphosphate (UTP)	≤0.5%
5-Bromouridine Diphosphate (5-Br-UDP)	≤0.3%
Water (KF)	≤5.0%
pH (10 mg/mL, aqueous)	7.0–8.5
Endotoxin	≤0.1 EU/μg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.