Description

Naphthol As-Bi n-Acetyl-β-D-Glucosaminide CAS NO 3395-37-7 is a specialized chromogenic substrate used in enzymatic histochemistry and cytochemistry. This compound is critical for the specific and sensitive detection of N-acetyl-β-D-glucosaminidase (NAGase) activity in biological tissues and cells. It is primarily utilized by researchers and diagnostic laboratories in life sciences, pathology, and biochemistry for studying lysosomal function and enzyme localization. The product offers high specificity and yields a stable, insoluble colored precipitate upon enzymatic cleavage, enabling precise microscopic analysis.

Application

• Histochemical Staining: Primary substrate for the localization and visualization of N-acetyl-β-D-glucosaminidase activity in tissue sections.
• Cytochemical Analysis: Used in cell smears and cultures to study lysosomal enzyme distribution and activity at the cellular level.
• Diagnostic Pathology: Aids in the research and diagnosis of lysosomal storage disorders and other conditions linked to altered NAGase levels.
• Enzyme Kinetics Research: Serves as a specific substrate for in vitro assays to study NAGase enzyme kinetics, inhibition, and purification.
• Biomedical Research: Employed in studies of inflammation, cellular differentiation, and apoptosis where lysosomal enzyme activity is a marker.
• Plant & Microbiology Studies: Applicable for detecting chitinase-related enzymatic activity in plant pathology and microbiological research.

Basic Information

Product Name	Naphthol As-Bi n-Acetyl-β-D-Glucosaminide
CAS No.	3395-37-7
Molecular Formula	C25H27N3O8
Molecular Weight	497.50 g/mol
Synonyms	Naphthol AS-BI N-acetyl-β-D-glucosaminide; 6-Bromo-2-hydroxy-3-naphthoic acid 2'-methoxy-4'-nitroanilide N-acetyl-β-D-glucosaminide; Naphthol AS-BI N-acetyl-β-D-glucosaminide; NAGase Substrate; Naphthol AS-BI β-D-Glucosaminide, N-acetyl-; 3-[(4-Nitro-2-methoxyphenyl)carbamoyl]-2-naphthyl 2-acetamido-2-deoxy-β-D-glucopyranoside; AS-BI NAG; Histochemical substrate for N-acetyl-β-glucosaminidase.
EINECS	Contact for details

Quality Control

Our Naphthol As-Bi n-Acetyl-β-D-Glucosaminide is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive histochemical applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification, to confirm its suitability as a diagnostic reagent. Certificates of Analysis (COA) detailing purity, identity, and performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation due to moisture absorption.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Performance	Suitable for histochemical use

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.