Description

4-Methylumbelliferyl Phosphate is a high-purity fluorogenic substrate and a critical reagent in enzymatic assays. Its primary value lies in its ability to generate a highly fluorescent product upon dephosphorylation, enabling sensitive detection and quantification of enzyme activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science research sectors, particularly for phosphatase and immunoassay applications.

Application

• Substrate for Alkaline Phosphatase (ALP): Widely used as a fluorogenic substrate in ELISA, Western blotting, and other immunoassays for signal amplification and detection.
• Enzyme Kinetics & Inhibition Studies: Employed in high-throughput screening (HTS) and biochemical research to study phosphatase enzyme kinetics and screen for potential inhibitors.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring ALP activity in serum, a marker for liver and bone disorders.
• Cell & Tissue Staining: Used in histochemistry and cytochemistry for the localization of phosphatase activity within biological samples.
• Microbiology & Food Safety Testing: Applied in assays to detect specific bacterial enzymes or as part of microbial identification systems.
• Research Tool in Signal Transduction: Utilized in studies of cell signaling pathways where phosphatase activity plays a regulatory role.

Basic Information

Product Name	4-Methylumbelliferyl Phosphate
CAS No.	3368-04-5
Molecular Formula	C10H9O6P
Molecular Weight	256.15 g/mol
Synonyms	4-MUP; 4-Methylumbelliferyl phosphate disodium salt (common salt form); 4-Methyl-7-hydroxycoumarin phosphate; 7-Hydroxy-4-methylcoumarin phosphate; 4-Methylumbelliferone phosphate; Phosphoric acid mono-(4-methyl-2-oxo-2H-chromen-7-yl) ester; MUP; 4-Methylumbelliferyl phosphate, disodium salt tetrahydrate
EINECS	222-155-8

Quality Control

Our 4-Methylumbelliferyl Phosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive diagnostic and research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Fluorescence	Conforms to reference standard upon enzymatic cleavage

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.