Description

3-Indoxyl Phosphate Disodium Salt is a high-purity chromogenic substrate widely used in enzymatic assays. Its primary value lies in its role as a key reagent for the detection of alkaline phosphatase (ALP) activity, producing a blue indigo dye upon hydrolysis. This compound is essential for researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors, enabling sensitive detection in applications such as ELISA, Western blotting, and histochemistry.

Application

• Diagnostic Reagent: As a critical substrate for detecting alkaline phosphatase (ALP) activity in clinical and research immunoassays (e.g., ELISA).
• Molecular Biology: Used in Western blotting, Southern blotting, and Northern blotting for the colorimetric detection of nucleic acids and proteins.
• Histochemistry & Cell Staining: Employed in tissue sections and cell-based assays to localize enzyme activity with high specificity.
• Biochemical Research: A fundamental tool for studying enzyme kinetics, inhibition, and phosphatase-related signaling pathways.
• Pharmaceutical Testing: Utilized in quality control and drug discovery assays to screen for compounds that modulate phosphatase activity.
• Biosensor Development: Integrated into diagnostic devices and biosensor platforms for point-of-care testing.

Basic Information

Product Name	3-Indoxyl Phosphate Disodium Salt
CAS No.	3318-43-2
Molecular Formula	C8H6NNa2O4P
Molecular Weight	269.08 g/mol
Synonyms	Indoxyl Phosphate Disodium Salt; 5-Indoxyl Phosphate Disodium Salt; 3-Indolyl Phosphate Disodium Salt; BCIP Disodium Salt; 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (common related compound); Alkaline Phosphatase Substrate; X-Phosphate; 3-(Dihydrogen phosphate)indole disodium salt; Indol-3-yl phosphate disodium salt
EINECS	222-015-5

Quality Control

Our 3-Indoxyl Phosphate Disodium Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopy for identification, and stringent controls for moisture and endotoxin levels where applicable. We provide Certificates of Analysis (COA) with each shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 2.0%
Solubility	Soluble in water
Functional Test (Enzymatic)	Passes performance test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.