Description

5-Bromo-4-Chloro-3-Indolyl Acetate is a chromogenic substrate widely used in enzymatic assays and histochemical staining. This compound is critical for the detection and visualization of specific enzyme activities, particularly esterases, due to its ability to produce an insoluble, colored precipitate upon cleavage. It is an essential reagent for researchers and manufacturers in the life sciences, diagnostic kit production, and biochemical research sectors, where precise and reliable detection methods are paramount.

Application

• Enzymatic Substrate in Diagnostic Assays: Primary use as a chromogenic substrate for detecting esterase activity in clinical and research ELISA, Western blot, and other immunoassay formats.
• Histochemistry and Cytochemistry: Used for the localization of enzyme activity in tissue sections and cell cultures, aiding in pathological diagnosis and biological research.
• Microbiology and Cell Biology: Employed in bacterial colony screening (e.g., blue-white screening) and reporter gene assays to monitor gene expression and cellular processes.
• Biochemical Research: Serves as a key reagent in studying enzyme kinetics, inhibition, and the function of various hydrolases.
• Diagnostic Kit Manufacturing: A critical raw material for producing test kits used in medical, veterinary, and food safety diagnostics.
• Pharmaceutical Development: Utilized in high-throughput screening (HTS) platforms during drug discovery to identify potential therapeutic compounds.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl Acetate
CAS No.	3252-36-6
Molecular Formula	C10H7BrClNO2
Molecular Weight	288.52 g/mol
Synonyms	X-Acetate; BCIP Acetate; 5-Bromo-4-chloro-1H-indol-3-yl acetate; 3-Indolyl acetate, 5-bromo-4-chloro-; 4-Chloro-5-bromo-3-indolyl acetate; Magenta-Phos; BCIP; BCIP substrate
EINECS	221-849-7

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl Acetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and tests for residual solvents and heavy metals, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment to guarantee traceability, consistency, and reliability for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility	Soluble in DMF, DMSO; slightly soluble in alcohol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.