Description

4-Nitrophenyl-β-D-Galactopyranoside is a chromogenic substrate widely used in enzymatic assays. This compound matters for its high specificity and sensitivity in detecting and quantifying β-galactosidase activity. It is essential for researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic industries. The product is supplied to meet the stringent requirements of analytical and research applications.

Application

• Primary substrate for the colorimetric and spectrophotometric assay of β-galactosidase enzyme activity.
• Critical reagent in molecular biology and genetic research, including reporter gene assays (e.g., lacZ gene expression).
• Used in diagnostic kits for the detection of enzymatic markers in clinical samples.
• Quality control testing in the pharmaceutical and food industries for microbial contamination and fermentation process monitoring.
• Tool in high-throughput screening (HTS) platforms for drug discovery and enzyme inhibitor studies.
• Application in biochemical research for studying enzyme kinetics and mechanism.

Basic Information

Product Name	4-Nitrophenyl-β-D-Galactopyranoside
CAS No.	3150-24-1
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	p-Nitrophenyl β-D-galactopyranoside; PNPG; 4-Nitrophenyl β-D-galactoside; p-Nitrophenyl β-D-galactoside; β-Galactosidase substrate; ONPG analog; 4-NPG; 2-(4-Nitrophenoxy)-6-(hydroxymethyl)oxane-3,4,5-triol
EINECS	221-579-8

Quality Control

Our 4-Nitrophenyl-β-D-Galactopyranoside is manufactured under a quality management system and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency. Standard quality parameters include identification, assay (HPLC), and impurity profile. Certificates of Analysis (COA) detailing all test results are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed after opening to minimize exposure to moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Approx. 175-185°C (dec.)
Solubility	Soluble in water, DMSO, DMF
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.