Description

4-Methoxyphenyl β-D-Galactopyranoside is a specialized glycoside derivative widely used as a chromogenic and fluorogenic substrate in enzymatic assays. This compound is critical for research and diagnostic applications where precise detection of β-galactosidase activity is required. It serves as an essential biochemical tool for scientists and manufacturers in the pharmaceutical, biotechnology, and life science research sectors.

Application

• Enzymatic Substrate for β-Galactosidase: Primary use as a chromogenic substrate in biochemical assays to detect and quantify β-galactosidase enzyme activity.
• Molecular Biology Research: Used in reporter gene assays (e.g., lacZ gene expression) for studying gene regulation and promoter activity.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical microbiology and genetic testing.
• Cell Biology & Histochemistry: Employed in staining techniques to visualize β-galactosidase expression in tissues and cell cultures.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to identify modulators of enzyme activity.
• Food & Environmental Testing: Applied in assays to detect bacterial contamination (e.g., E. coli) which express β-galactosidase.

Basic Information

Product Name	4-Methoxyphenyl β-D-Galactopyranoside
CAS No.	3150-20-7
Molecular Formula	C₁₃H₁₈O₈
Molecular Weight	302.28 g/mol
Synonyms	p-Methoxyphenyl β-D-galactopyranoside; 4-Methoxyphenyl β-D-galactoside; p-Methoxyphenyl β-D-galactoside; 4-Methoxy-phenyl-β-D-galactopyranoside; β-D-Galactopyranoside, 4-methoxyphenyl; Gal-ONp-OMe; 4-Methoxy-β-D-galactopyranoside; ONPG-OMe
EINECS	221-579-8

Quality Control

Our 4-Methoxyphenyl β-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, NMR and IR for structural confirmation, and specific tests for residual solvents and moisture. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with research and diagnostic-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.