Description

6-FITC (CAS NO 3012-71-3) is a high-purity fluorescent labeling reagent, a derivative of fluorescein isothiocyanate (FITC) designed for advanced conjugation chemistry. This compound is critical for creating stable, fluorescently-tagged probes used in sensitive detection and imaging applications. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, flow cytometry, immunofluorescence, and diagnostic assay development.

Application

• Antibody and Protein Labeling: Conjugation to primary amines for fluorescence-activated cell sorting (FACS) and microscopy.
• Oligonucleotide and Nucleic Acid Probes: Fluorescent tagging of DNA/RNA for in situ hybridization (FISH) and real-time PCR applications.
• Diagnostic Kit Manufacturing: Key component in the production of lateral flow assays and ELISA kits for clinical diagnostics.
• Biomaterial and Polymer Labeling: Tracking and visualization of nanoparticles, hydrogels, and drug delivery systems.
• Cellular Imaging and Tracking: Long-term cell tracing and intracellular protein localization studies.
• Bioconjugation Research: Development of novel fluorescent biosensors and chemical biology tools.

Basic Information

Product Name	6-FITC
CAS No.	3012-71-3
Molecular Formula	C21H11NO5S
Molecular Weight	389.38 g/mol
Synonyms	6-Fluorescein Isothiocyanate; 6-Isothiocyanatofluorescein; FITC Isomer I; 5(6)-FITC; Fluorescein 5(6)-Isothiocyanate; 5(6)-Carboxyfluorescein N-succinimidyl ester precursor; 6-FITC isomer; Fluorescein isothiocyanate isomer I
EINECS	221-138-5

Quality Control

Our 6-FITC is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing purity, isomer content, and performance data is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0% (6-FITC isomer)
Total FITC Isomer Content	≥ 98.0%
Water Content (KF)	≤ 1.0%
Fluorescence	Conforms to reference standard
Functional Performance (Labeling)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.