Description

4-Methylumbelliferyl Phosphate is a high-purity fluorogenic substrate widely used as a critical reagent in enzymatic assays. Its core value lies in its ability to generate a highly fluorescent product upon enzymatic dephosphorylation, enabling sensitive and quantitative detection of enzyme activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science research sectors, particularly for phosphatase and immunoassay applications.

Application

• Substrate for Alkaline Phosphatase (ALP): Primary fluorogenic substrate for detecting and quantifying alkaline phosphatase activity in ELISA, Western blotting, and other immunoassays.
• Enzyme Kinetics & Inhibition Studies: Used in high-throughput screening (HTS) and biochemical research to study phosphatase enzyme kinetics and evaluate potential inhibitors.
• Diagnostic Reagent Manufacturing: A key raw material in the production of clinical diagnostic kits for measuring ALP levels, which are indicators of liver and bone disorders.
• Cell & Tissue Staining: Employed in histochemistry and cytochemistry for the localization of phosphatase activity within biological samples.
• Microbiology & Food Safety Testing: Used in assays to detect bacterial contamination, as certain bacteria produce phosphatases that can be detected with this substrate.
• Research Tool in Signal Transduction: Applied in studies of cell signaling pathways that involve protein phosphatases.

Basic Information

Product Name	4-Methylumbelliferyl Phosphate
CAS No.	336-84-5
Molecular Formula	C10H9O6P
Molecular Weight	256.15 g/mol
Synonyms	4-MUP; 4-Methylumbelliferyl phosphate disodium salt; 4-Methylumbelliferyl phosphate, disodium salt; 7-Hydroxy-4-methylcoumarin phosphate; 4-Methyl-7-hydroxycoumarin phosphate; Phosphoric acid mono-(4-methyl-2-oxo-2H-chromen-7-yl) ester; 4-Methylumbelliferyl dihydrogen phosphate; 4-MU-P
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl Phosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Fluorescence	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.