Description

p-Nitrophenyl Phosphate Di(Tris) Salt is a high-purity chromogenic substrate widely used in enzymatic assays. This compound is critical for detecting and quantifying the activity of alkaline phosphatase and other phosphatases, serving as a key reagent in diagnostic and research applications. It is essential for manufacturers and research institutions in the in-vitro diagnostics (IVD), life science research, and biotechnology sectors who require reliable and sensitive substrates for colorimetric detection.

Application

• Primary Substrate for Alkaline Phosphatase (ALP): Used as the key chromogenic component in ELISA kits, immunoblotting (western blot), and other immunoassays for signal generation.
• Clinical Diagnostics: Integral to automated clinical chemistry analyzers for measuring ALP activity in human serum, a vital marker for liver and bone disorders.
• Molecular Biology Research: Employed in nucleic acid detection systems, such as non-radioactive labeling and detection for Southern, Northern, and colony blot hybridization.
• Enzyme Activity Studies: Used in biochemical research to study enzyme kinetics, inhibition, and purification of various phosphatases.
• Point-of-Care Testing (POCT): Formulated into dry chemistry strips and lateral flow assays for rapid, on-site diagnostic testing.
• Pharmaceutical Quality Control: Applied in potency assays for biopharmaceuticals where alkaline phosphatase is used as a conjugate or reporter enzyme.

Basic Information

Product Name	p-Nitrophenyl Phosphate Di(Tris) Salt
CAS No.	330-13-2
Molecular Formula	C9H14NO8P
Molecular Weight	295.18 g/mol
Synonyms	PNPP Di(Tris) Salt; 4-Nitrophenyl Phosphate Di(Tris) Salt; pNPP; Phosphoric Acid 4-Nitrophenyl Ester Di(Tris) Salt; Tris p-Nitrophenyl Phosphate; ALP Substrate; Alkaline Phosphatase Substrate; Diagnostic Grade PNPP; 4-NPP Di(Tris) Salt
EINECS	206-347-5

Quality Control

Our p-Nitrophenyl Phosphate Di(Tris) Salt is manufactured under strict quality systems to ensure batch-to-batch consistency suitable for diagnostic and research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for molar absorptivity, and enzymatic activity validation. Certificates of Analysis (COA) detailing all specifications are provided and traceable to reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is kept sealed to prevent absorption of atmospheric moisture, which can affect performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance Ratio (A400nm/A310nm)	≤ 0.05
Enzymatic Suitability (ALP)	Conforms (Kinetic Test)
Solubility (1M Diethanolamine Buffer)	Clear, colorless solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.