Description

5-Azacytidine is a potent cytidine analog and a well-characterized DNA methyltransferase inhibitor, widely recognized for its role in epigenetic research and therapeutic development. Its primary value lies in its ability to induce DNA demethylation and reactivate silenced genes, making it a critical tool for studying cellular differentiation, reprogramming, and cancer biology. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors focused on oncology, stem cell research, and the development of novel epigenetic therapies.

Application

• Epigenetics & Cancer Research: As a DNA methyltransferase inhibitor, it is used to study gene silencing, tumor suppressor gene reactivation, and mechanisms of carcinogenesis.
• Stem Cell & Developmental Biology: Employed to investigate cellular reprogramming, induced pluripotent stem (iPS) cell generation, and differentiation pathways.
• Pharmaceutical Development: Serves as an active pharmaceutical ingredient (API) and a reference standard in the development of myelodysplastic syndrome (MDS) and leukemia treatments.
• Biochemical Assays: Used as a key reagent in high-throughput screening and in vitro assays targeting epigenetic enzymes.
• Cell Culture Studies: Applied in vitro to modulate gene expression profiles and study the effects of DNA demethylation on various cell lines.

Basic Information

Product Name	5-Azacytidine
CAS No.	320-67-2
Molecular Formula	C8H12N4O5
Molecular Weight	244.20 g/mol
Synonyms	5-Azacytidine; Azacitidine; 5-AZC; Ladakamycin; 4-Amino-1-β-D-ribofuranosyl-1,3,5-triazin-2(1H)-one; 1-β-D-Ribofuranosyl-4-amino-1,3,5-triazin-2(1H)-one; NSC-102816; Vidaza (brand name)
EINECS	206-274-9

Quality Control

Our 5-Azacytidine is manufactured under strict quality management systems to ensure high purity and batch-to-batch consistency, suitable for research and pharmaceutical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We can supply material that meets relevant standards for research use and can provide support for applications requiring compliance with cGMP or other regulatory frameworks.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Related Substances (HPLC)	Total impurities ≤ 2.0%
Bacterial Endotoxins	Contact for details
Microbial Enumeration	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.