Description

(+/-)-Muscarine Chloride CAS NO 300-54-9 is a high-purity, racemic form of the classic muscarinic acetylcholine receptor agonist. This compound is essential for neuroscience and pharmacological research, serving as a critical reference standard and tool compound. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and academic sectors for receptor binding studies, assay validation, and the development of cholinergic therapeutics.

Application

• Pharmacological Research: As a potent agonist for studying muscarinic acetylcholine receptor (mAChR) subtypes (M1-M5) in vitro and in vivo.
• Neuroscience Tool Compound: For investigating cholinergic signaling pathways, synaptic transmission, and the role of mAChRs in cognitive and peripheral nervous system functions.
• Drug Discovery & Development: Used as a reference standard in high-throughput screening (HTS) assays to identify and characterize new mAChR modulators, antagonists, or allosteric regulators.
• Biochemical Assay Validation: Serves as a positive control in functional assays (e.g., calcium flux, IP1 accumulation) and radioligand binding assays to ensure assay performance and reproducibility.
• Academic & Institutional Research: Fundamental studies in neuropharmacology, toxicology, and physiology to understand the effects of cholinergic activation.
• Quality Control & Reference Standard: Used by manufacturers of fine chemicals and active pharmaceutical ingredients (APIs) as an analytical reference material for purity and identity confirmation.

Basic Information

Item	Detail
Product Name	(+/-)-Muscarine Chloride
CAS No.	300-54-9
Molecular Formula	C9H21ClNO2 +
Molecular Weight	210.72 g/mol (as chloride salt)
Synonyms	Muscarine Chloride (racemic); DL-Muscarine Chloride; (±)-Muscarine Chloride; (2S,4R,5S)-(±)-Muscarine Chloride; (4-Hydroxy-5-methyl-2-tetrahydrofuranyl)methyl-trimethylammonium Chloride; O-Acetylcholine Chloride (obsolete); L(+)-Muscarine Chloride (for one enantiomer); Agonist of muscarinic acetylcholine receptors
EINECS	206-101-5

Quality Control

Our (+/-)-Muscarine Chloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and residual solvent analysis, to ensure it meets the stringent requirements for research and reference standards. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all test results against established specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant in the container. Keep under inert atmosphere if specified for high-purity grades.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Specific Rotation (if applicable)	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.