Description

Biotin-Peg3-Ss-Azide CAS NO 2866429-93-6 is a heterobifunctional crosslinker designed for advanced bioconjugation and click chemistry applications. This compound integrates biotin for affinity capture, a cleavable disulfide (Ss) bridge for controlled release, and an azide group for copper-free strain-promoted alkyne-azide cycloaddition (SPAAC). It is essential for researchers and developers requiring precise, reversible, and bioorthogonal labeling of biomolecules. Primary users include pharmaceutical R&D teams, diagnostic kit manufacturers, and academic laboratories focused on proteomics, drug delivery systems, and biomarker discovery.

Application

• Proteomics & Biomarker Discovery: Site-specific labeling of proteins and peptides for affinity purification and mass spectrometry analysis.
• Antibody-Drug Conjugate (ADC) Development: Facilitating the construction of cleavable linker-payload systems for targeted cancer therapies.
• Diagnostic Assay Development: Conjugation of detection probes (e.g., fluorescent dyes, enzymes) to antibodies or oligonucleotides with a reversible linkage.
• Cell Surface Engineering: Bioorthogonal labeling of cell surface glycans or proteins for imaging and tracking studies.
• Polymer & Material Science: Functionalization of nanoparticles, hydrogels, or other polymeric materials with bioactive ligands.
• Pull-Down Assays: Immobilization of biotinylated targets onto streptavidin resins with the option for gentle, reducing agent-mediated elution.
• Drug Delivery System Research: Creating stimuli-responsive nanocarriers where the disulfide bond cleaves in the reductive intracellular environment.

Basic Information

Product Name	Biotin-Peg3-Ss-Azide
CAS No.	2866429-93-6
Molecular Formula	C₂₆H₄₆N₈O₇S₂
Molecular Weight	638.83 g/mol
Synonyms	Biotin-PEG3-SS-Azide; Biotin-PEG3-disulfide-azide; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(14-azido-3,6,9,12-tetraoxatetradecyl)-4-oxopentanamide; 1-Biotinamido-3,6,9,12-tetraoxapentadecan-15-yl 3-((2-(pyridin-2-yldisulfanyl)ethyl)amino)-3-oxopropylcarbamate; Azide-PEG3-SS-Biotin; SS-Biotin-PEG3-N3; Biotinylated cleavable PEG linker, azide-terminated
EINECS	Contact for details

Quality Control

Every batch of Biotin-Peg3-Ss-Azide is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data for purity (HPLC), identity confirmation (NMR, MS), and functional group integrity. Our quality protocols are designed to support research and development under GMP/GLP-like guidelines, ensuring consistency and reliability for critical bioconjugation workflows.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Store away from strong reducing agents.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.