Description

Dbco-Peg3-Acid CAS NO 2754384-59-1 is a heterobifunctional crosslinker featuring a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) and a carboxylic acid for further conjugation. This compound is essential for creating stable, catalyst-free bioconjugates in demanding research and development environments. It is a critical reagent for scientists in biopharmaceuticals, diagnostics, and advanced materials science who require efficient and bioorthogonal labeling strategies.

Application

• Antibody-Drug Conjugate (ADC) Linker: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via the carboxylic acid group, utilizing the DBCO for bioorthogonal coupling to azide-functionalized proteins.
• Fluorescent Probe & Diagnostic Reagent Synthesis: Conjugation of dyes, quenchers, or other reporter molecules to targeting ligands (peptides, antibodies, oligonucleotides) for imaging and detection assays.
• Polymer & Biomaterial Functionalization: Introducing click-reactive DBCO handles onto polymer backends or hydrogel matrices for subsequent modification with azide-bearing biomolecules.
• Surface Immobilization & Microarray Fabrication: Creating functionalized surfaces for protein or cell capture studies by reacting the acid group with amine-coated slides, followed by DBCO-mediated immobilization.
• Peptide & Protein Labeling: Efficient, copper-free modification of azide-tagged proteins or peptides for structural studies, pull-down assays, or tracking.
• Nanoparticle & Liposome Conjugation: Functionalizing nanocarriers with targeting ligands or stealth polymers to enhance drug delivery specificity and circulation time.

Basic Information

Product Name	Dbco-Peg3-Acid
CAS No.	2754384-59-1
Molecular Formula	C27H29NO7
Molecular Weight	479.52 g/mol
Synonyms	DBCO-PEG3-COOH; DBCO-PEG3-Acid; DBCO-PEG3-Acid; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (2,5,8,11-tetraoxatetradecan-14-yl)carbamate; 2,5,8,11-Tetraoxatetradecan-14-oic acid, 14-[[(1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethyl]carbamate]; Dibenzocyclooctyne-PEG3-acid; DBCO-PEG3-Propionic Acid; Azadibenzocyclooctyne-PEG3-acid
EINECS	Contact for details

Quality Control

Our Dbco-Peg3-Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your research and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.