Description

Spdp-Peg12-Nhs Ester is a heterobifunctional crosslinker featuring an amine-reactive NHS ester and a sulfhydryl-reactive pyridyldithiol group, connected by a flexible, hydrophilic PEG12 spacer. This compound is critical for creating stable, site-specific conjugations between proteins, peptides, or other biomolecules, enabling advanced research and development in bioconjugation. It is an essential reagent for scientists and manufacturers in the fields of biopharmaceuticals, antibody-drug conjugate (ADC) development, and diagnostic assay formulation who require precise and controllable linking chemistry.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key building block for constructing cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein-Protein Conjugation: For creating defined protein complexes, fusion proteins, or attaching functional tags (e.g., fluorescent dyes, enzymes) to specific sites.
• Surface Functionalization: Modifying nanoparticles, biosensors, or chromatography matrices with thiol-reactive groups for subsequent biomolecule immobilization.
• PEGylation Reagent: Introducing a long, hydrophilic polyethylene glycol (PEG) chain to biomolecules to enhance solubility, stability, and circulation half-life.
• Diagnostic Reagent Synthesis: Developing enzyme-linked or fluorescent immunoassays by conjugating detection enzymes or probes to antibodies or antigens.
• Biomaterial Engineering: Crosslinking hydrogels or functionalizing polymeric materials for controlled drug delivery or tissue engineering applications.

Basic Information

Product Name	Spdp-Peg12-Nhs Ester
CAS No.	2601437-72-1
Molecular Formula	C46H78N4O19S2
Molecular Weight	1055.27 g/mol
Synonyms	SPDP-PEG12-NHS ester; NHS-PEG12-SPDP; N-Succinimidyl 3-((2-pyridyldithio)propionyl)-PEG12-propionate; Succinimidyl-[(N-maleimidopropionamido)-dodecaethyleneglycol] ester; 3-(2-Pyridyldithio)propionyl-PEG12-NHS ester; SPDP-PEG12-OSu; Heterobifunctional PEG crosslinker, NHS-PDP
EINECS	Contact for details

Quality Control

Our Spdp-Peg12-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and structural confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.