Description

Acid-Peg25-Nhs Ester CAS NO 2563872-17-1 is a heterobifunctional polyethylene glycol (PEG) derivative featuring a carboxylic acid group and an N-hydroxysuccinimide (NHS) ester at opposite termini. This compound is engineered to facilitate the controlled, covalent conjugation of biomolecules, such as peptides, proteins, and antibodies, to surfaces, particles, or other molecules. It is an essential reagent for researchers and manufacturers in the fields of bioconjugation, diagnostics, and advanced drug delivery systems, where precise and stable linkages are required.

Application

• Bioconjugation Reagent: For the covalent coupling of proteins, antibodies, or peptides to nanoparticles, surfaces, or other biomolecules via the NHS ester and acid groups.
• PEGylation Linker: Used to introduce a hydrophilic PEG spacer to drugs or proteins, improving solubility, stability, and pharmacokinetics.
• Diagnostic Assay Development: Employed in the fabrication of biosensors and immunoassay components by immobilizing capture molecules onto solid supports.
• Drug Delivery System Fabrication: A key building block for creating targeted therapeutic conjugates, such as antibody-drug conjugates (ADCs).
• Surface Functionalization: Modifying materials (e.g., gold, silica, polymers) with a reactive handle for subsequent biomolecule attachment in tissue engineering and biomaterials research.
• Crosslinking Agent: For creating stable bridges between amine-containing and carboxyl-containing molecules in complex hydrogel or polymer networks.

Basic Information

Product Name	Acid-Peg25-Nhs Ester
CAS No.	2563872-17-1
Molecular Formula	Contact for details
Molecular Weight	~1,200 - 1,300 g/mol (Average)
Synonyms	α-Acid-ω-NHS-PEG25; NHS-PEG25-Acid; Carboxy-PEG25-NHS ester; N-Hydroxysuccinimide ester of PEG25 carboxylic acid; HOOC-PEG25-NHS; NHS-PEG25-COOH; PEG25 NHS Acid Ester; Succinimidyl ester of PEG25 acid
EINECS	Contact for details

Quality Control

Our Acid-Peg25-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and NMR for structural confirmation, to ensure consistency and performance. Certificates of Analysis (COA) detailing purity, functional group substitution, and critical specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous liquid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Average Molecular Weight	As per theoretical value ± 5%
Functional Substitution (Acid)	≥ 90%
Functional Substitution (NHS Ester)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.