Description

20-Hete-D6 is a deuterium-labeled analog of 20-Hydroxyeicosatetraenoic acid (20-HETE), a key eicosanoid metabolite of arachidonic acid. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry-based analytical methods, ensuring unparalleled accuracy and precision in measurement. It is critically needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and academic sectors for advanced metabolomics studies, drug metabolism and pharmacokinetics (DMPK) research, and biomarker discovery.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical internal standard for the accurate quantification of endogenous 20-HETE levels in complex biological matrices such as plasma, serum, urine, and tissue homogenates.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in pharmaceutical R&D to study the metabolic pathways and pharmacokinetic profiles of drug candidates that interact with the cytochrome P450 (CYP) enzyme pathways responsible for 20-HETE synthesis.
• Biomarker Research & Clinical Diagnostics: Enables precise measurement of 20-HETE, a bioactive lipid implicated in cardiovascular regulation, hypertension, renal function, and cancer, for use as a potential clinical biomarker.
• Metabolomics and Lipidomics Studies: A vital tool in high-throughput profiling to understand the role of eicosanoids in disease states, enabling differentiation between diseased and healthy metabolic states.
• Enzyme Activity Assays: Employed in in vitro assays to measure the activity of CYP4A and CYP4F enzymes, which are responsible for 20-HETE production.
• Quality Control & Method Validation: Essential for developing, validating, and ensuring the robustness and regulatory compliance (e.g., FDA, EMA) of bioanalytical methods in contract research organizations (CROs) and pharmaceutical QC labs.

Basic Information

Product Name	20-Hete-D6
CAS No.	2548939-89-3
Molecular Formula	C20H26D6O3
Molecular Weight	322.48 g/mol (approx.)
Synonyms	20-Hydroxyeicosatetraenoic acid-d6; 20-HETE-d6; 20-Hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic-20,20,21,21,22,22-d6 acid; Deuterated 20-HETE; [20,20,21,21,22,22-2H6]-20-Hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid; 20(S)-HETE-d6; 20(R)-HETE-d6; Arachidonic acid metabolite-d6 (20-HETE)
EINECS	Contact for details

Quality Control

Our 20-Hete-D6 is manufactured under strict quality-controlled conditions to ensure the highest isotopic purity and chemical integrity required for sensitive analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing specifications for isotopic purity (typically >98 atom % D), chemical purity (by HPLC), and confirmation of structure (by NMR and MS). We support research and development requiring compliance with GLP and GMP guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store under an inert atmosphere (e.g., argon or nitrogen) in a sealed, desiccated environment. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Colorless to pale yellow oil or film
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Isotopic Purity	≥98 atom % D
Chemical Purity (HPLC)	≥95%
Isomeric Purity (S/R ratio)	Contact for details
Solvent Content (Residual)	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.