Description

Dbco-Peg1-Nh-Boc CAS NO 2364591-77-3 is a heterobifunctional crosslinker featuring a strained alkyne (DBCO) for copper-free click chemistry and a Boc-protected amine for subsequent conjugation. This compound matters for its role in enabling precise, efficient, and biocompatible bioconjugation strategies, which are critical for developing advanced therapeutics and diagnostics. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors for applications such as antibody-drug conjugate (ADC) development, protein labeling, and surface functionalization of nanoparticles and biomaterials.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to antibodies via the DBCO group, followed by deprotection and functionalization of the amine.
• Protein and Peptide Labeling: Modification of azide-functionalized proteins or peptides for fluorescence, biotinylation, or immobilization studies.
• Surface Functionalization: Crafting functional surfaces on nanoparticles, biosensors, or microarray slides for specific biomolecular capture.
• Polymer and Dendrimer Synthesis: Serving as a key building block for creating functionalized, branched macromolecular structures.
• Prodrug Synthesis: Constructing cleavable linkers in prodrug systems where the Boc-protected amine provides a handle for attaching targeting moieties or trigger mechanisms.
• Chemical Biology Probes: Creating activity-based probes for studying enzyme function or cellular pathways using bioorthogonal chemistry.
• Hydrogel Formation: Cross-linking azide-modified polymers to form hydrogels for 3D cell culture or drug delivery matrices.
• Diagnostic Assay Development: Conjugating detection molecules (e.g., enzymes, dyes) to solid supports or other reagents in immunoassays.

Basic Information

Product Name	Dbco-Peg1-Nh-Boc
CAS No.	2364591-77-3
Molecular Formula	C₂₆H₂₈N₂O₅
Molecular Weight	448.51 g/mol
Synonyms	DBCO-PEG1-NH-Boc; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-(2-((tert-butoxycarbonyl)amino)ethyl)carbamate; Dibenzocyclooctyne-PEG1-Amine-Boc; DBCO-PEG1-Amine-Boc; DBCO-NH-PEG1-Boc; tert-Butyl (2-(((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethoxy)carbonylamino)ethyl)carbamate; BCN-PEG1-NH-Boc (Note: BCN is a common synonym for DBCO); Azadibenzocyclooctyne-PEG1-Amine-Boc
EINECS	Contact for details

Quality Control

Every batch of Dbco-Peg1-Nh-Boc undergoes rigorous analytical testing to ensure identity, purity, and performance consistency suitable for research and bioconjugation applications. Our standard quality commitment includes comprehensive analysis by HPLC, NMR, and mass spectrometry. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (by HPLC)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.