Description

Tco-Peg12-Tfp Ester is a specialized heterobifunctional polyethylene glycol (PEG) crosslinker featuring a light-cleavable Tco (trans-Cyclooctene) group and a highly reactive Tfp (Tetrafluorophenyl) ester terminus. This compound is engineered for advanced bioconjugation strategies, enabling controlled, site-specific coupling of biomolecules under mild, aqueous conditions. It is an essential reagent for researchers and developers in pharmaceutical R&D, proteomics, and diagnostic assay development, where precise and reversible linkage is required.

Application

• Bioorthogonal Chemistry & Click Chemistry: Utilized for inverse electron-demand Diels-Alder (IEDDA) reactions with tetrazines for rapid, catalyst-free labeling and conjugation.
• Antibody-Drug Conjugate (ADC) Development: Serves as a key linker for attaching cytotoxic payloads to monoclonal antibodies, leveraging the cleavable Tco group for controlled drug release.
• Protein and Peptide Modification: Enables site-specific PEGylation or functionalization of proteins via reaction with primary amines, enhancing stability and pharmacokinetics.
• Surface Functionalization: Used to create bioactive surfaces on chips, beads, or nanoparticles for biosensor and microarray applications.
• Fluorescent Probe & Diagnostic Reagent Synthesis: Acts as a linker for conjugating dyes, quenchers, or other reporter molecules to targeting ligands.
• Materials Science: Incorporated into hydrogels or polymer networks to create stimuli-responsive biomaterials that degrade upon light exposure.

Basic Information

Item	Detail
Product Name	Tco-Peg12-Tfp Ester
CAS No.	2353410-07-6
Molecular Formula	C45H70F4N2O16
Molecular Weight	971.1 g/mol (Contact for details)
Synonyms	trans-Cyclooctene-PEG12-Tetrafluorophenyl Ester; TCO-PEG12-TFPE; (E)-Cyclooct-4-enyl 3,6,9,12,15,18,21,24,27,30,33,36-dodecaoxaoctatriacontan-38-oate 2,3,5,6-tetrafluorophenyl ester; TCO-PEG12-TFP Ester; TCO-PEG12-TFP; Heterobifunctional PEG Crosslinker TCO-PEG-TFP; TCO-PEG12-OSu (related compound); TCO-PEG12-NHS Ester (related compound)
EINECS	Contact for details

Quality Control

Every batch of Tco-Peg12-Tfp Ester is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure high purity and batch-to-batch consistency, critical for sensitive bioconjugation workflows. Certificates of Analysis (COA) detailing purity, identity, and functional group integrity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR / NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Functional Group)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, and dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.