Description

Tco-Peg8-Nhs Ester CAS NO 2353409-95-5 is a heterobifunctional crosslinking reagent featuring a trans-cyclooctene (TCO) group for bioorthogonal click chemistry and an amine-reactive N-hydroxysuccinimide (NHS) ester, connected by a hydrophilic polyethylene glycol (PEG8) spacer. This compound is engineered to enable rapid, specific, and catalyst-free conjugation of biomolecules, such as antibodies, peptides, or proteins, to tetrazine-modified surfaces, nanoparticles, or other probes via the inverse electron-demand Diels-Alder (IEDDA) reaction. It is an essential tool for researchers and developers in bioconjugation, diagnostic assay development, targeted drug delivery systems, and advanced materials science, where efficient and stable labeling under physiological conditions is required.

Application

• Antibody-Drug Conjugate (ADC) Linker Synthesis: For constructing cleavable or non-cleavable linkers between cytotoxic payloads and targeting antibodies.
• Diagnostic Probe Labeling: Conjugation of fluorescent dyes, enzymes, or other detection moieties to antibodies or proteins for ELISA, flow cytometry, or in vivo imaging.
• Surface Functionalization: Modification of nanoparticles, quantum dots, or microarrays with targeting ligands for biosensing and cellular targeting applications.
• Pretargeted Imaging and Therapy: A key component in pretargeting strategies for nuclear medicine (e.g., PET, SPECT) and radiotherapy to improve target-to-background ratios.
• Hydrogel and Biomaterial Crosslinking: Incorporation into polymer networks to create functionalized biomaterials for tissue engineering and controlled release.
• Protein-Polymer Conjugation: Site-specific PEGylation of therapeutic proteins to enhance pharmacokinetic properties like solubility and circulation half-life.
• Chemical Biology Tool Development: Used to create activity-based probes for studying enzyme function or protein-protein interactions in live cells.

Basic Information

Product Name	Tco-Peg8-Nhs Ester
CAS No.	2353409-95-5
Molecular Formula	C₃₇H₆₀N₂O₁₃
Molecular Weight	752.9 g/mol
Synonyms	NHS-PEG8-TCO; TCO-PEG8-NHS Ester; trans-Cyclooctene-PEG8-NHS; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-((2,5-dioxopyrrolidin-1-yl)oxy)-1-oxo-3,6,9,12,15,18,21,24-octaoxahexacosan-26-yl) carbamate; TCO-PEG8-NHS; TCO-PEG₈-NHS Ester; 8-Arm PEG TCO NHS Ester; Heterobifunctional PEG Crosslinker TCO-NHS
EINECS	Contact for details

Quality Control

Our Tco-Peg8-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment. We support development under cGMP guidelines for pre-clinical and clinical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep under an inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.