Description

Gly-Gly-Gly-Peg4-Methyltetrazine is a specialized, multifunctional chemical linker that combines a tri-glycine peptide sequence with a polyethylene glycol (PEG) spacer and a bioorthogonal methyltetrazine group. This compound matters for its unique ability to facilitate precise, efficient, and stable conjugation in aqueous, biological environments, enabling advanced research and development. It is primarily needed by scientists and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, proteomics, and the creation of targeted diagnostics and therapeutics.

Application

• Bioconjugation & ADC Linker: Serves as a cleavable or non-cleavable linker for constructing antibody-drug conjugates (ADCs) and other protein-payload constructs.
• Bioorthogonal Labeling: Utilizes the methyltetrazine group for rapid, specific, and catalyst-free inverse electron-demand Diels-Alder (IEDDA) reactions with strained alkenes/alkynes (e.g., trans-cyclooctene) for live-cell imaging and labeling.
• Peptide & Protein Modification: Enables site-specific modification of peptides, proteins, and antibodies via the reactive tetrazine moiety and the peptide backbone.
• Proteomics Research: Used as a tool compound for crosslinking studies, affinity purification, and mapping protein-protein interactions.
• Drug Delivery Systems: Incorporated into nanocarriers and prodrugs to create targeted delivery platforms with enhanced solubility and pharmacokinetics due to the PEG spacer.
• Diagnostic Probe Development: Facilitates the attachment of imaging agents (e.g., fluorophores, radionuclides) to targeting molecules for in vitro and in vivo diagnostics.
• Materials Science & Surface Functionalization: Applied to modify surfaces and materials with bioactive peptides for cell culture, biosensors, and tissue engineering.

Basic Information

Product Name	Gly-Gly-Gly-Peg4-Methyltetrazine
CAS No.	2353409-82-0
Molecular Formula	C30H48N10O11
Molecular Weight	700.77 g/mol
Synonyms	Gly-Gly-Gly-PEG4-Methyltetrazine; Tri-Glycine-PEG4-Methyltetrazine; GGG-PEG4-MeTz; (Gly)3-PEG4-Methyltetrazine; N-(Methyltetrazinyl-PEG4)-tri-glycine; 5-((1-(13,16,19,22-Tetraoxa-4,7-dioxo-10-azatetracosan-1-yl)-1H-1,2,3-triazol-4-yl)methyl)-1-methyl-1H-tetrazole; Methyltetrazine-PEG4-GGG; Tetrazine-PEG4-Gly-Gly-Gly
EINECS	Contact for details

Quality Control

Our Gly-Gly-Gly-Peg4-Methyltetrazine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive research and development applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.