Description

n-Desthiobiotin-n-Bis(Peg4-Nhs Ester) CAS NO 2353409-61-5 is a specialized, heterobifunctional crosslinking reagent designed for advanced bioconjugation and labeling applications. This compound is engineered to facilitate the stable and site-specific attachment of biomolecules, such as proteins and antibodies, to various surfaces, probes, or other molecules. It is an essential tool for researchers and developers in the fields of biopharmaceuticals, diagnostic assay development, and proteomics, where precise and reliable conjugation is critical.

Application

• Antibody-Drug Conjugate (ADC) Development: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies.
• Diagnostic Assay Reagent: Used in the development of ELISA, lateral flow assays, and other immunoassays for labeling detection antibodies.
• Protein Immobilization: Enables the covalent attachment of proteins to biosensor chips, microarray slides, or chromatography resins.
• Fluorescent Probe Synthesis: Acts as a linker for attaching fluorophores to targeting molecules for cellular imaging and flow cytometry.
• Streptavidin/Biotin System Enhancement: Provides a cleavable or extended PEG spacer arm for modifying biotin-binding interactions in pull-down assays and purification.
• Polymer-Protein Conjugate Synthesis: Facilitates the PEGylation of therapeutic proteins to improve pharmacokinetic profiles.
• Surface Functionalization: Used to create bioactive coatings on nanoparticles or medical devices.

Basic Information

Product Name	n-Desthiobiotin-n-Bis(Peg4-Nhs Ester)
CAS No.	2353409-61-5
Molecular Formula	C44H72N6O20
Molecular Weight	1005.1 g/mol
Synonyms	Desthiobiotin-PEG4-NHS ester; Bis-NHS-PEG4-desthiobiotin; n-Desthiobiotin-n-Bis(PEG4-NHS ester); 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[3-(5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanamido]propanamido]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate; PEG4-Desthiobiotin-Bis-NHS; Heterobifunctional PEG Crosslinker NHS-Desthiobiotin
EINECS	Contact for details

Quality Control

Every batch of n-Desthiobiotin-n-Bis(Peg4-Nhs Ester) is manufactured and tested under strict quality management systems. Our products undergo rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high purity and consistent performance for sensitive bioconjugation workflows. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.