Description

Diketone-Peg12-Biotin CAS NO 2353409-53-5 is a high-purity, heterobifunctional crosslinking agent featuring a diketone group for site-specific conjugation and a biotin moiety for affinity purification or detection. This compound is engineered to enable precise and stable bioconjugation strategies, particularly valuable for creating advanced biotherapeutics and diagnostic reagents. It is an essential tool for researchers and manufacturers in the fields of bioconjugation chemistry, antibody-drug conjugate (ADC) development, and targeted drug delivery systems.

Application

• Antibody-Drug Conjugate (ADC) Linker: Site-specific conjugation of cytotoxic payloads to antibodies via engineered ketone-containing amino acids.
• Protein and Peptide Labeling: Functionalization of biomolecules with biotin tags for purification (e.g., streptavidin affinity columns) or detection (e.g., ELISA, flow cytometry).
• Surface Immobilization: Crafting functionalized surfaces for biosensor development, microarray platforms, and diagnostic assay substrates.
• Polymer-Protein Conjugation: Synthesis of PEGylated biotherapeutics to enhance pharmacokinetic properties such as solubility and circulation half-life.
• Small Molecule-Protein Conjugates: Development of hapten-carrier protein complexes for vaccine and immunology research.
• Diagnostic Reagent Synthesis: Production of labeled antibodies, antigens, and other detection molecules for in vitro diagnostics (IVD).

Basic Information

Product Name	Diketone-Peg12-Biotin
CAS No.	2353409-53-5
Molecular Formula	C58H100N6O20S
Molecular Weight	1233.5 g/mol
Synonyms	Biotin-PEG12-Diketone; Diketone-PEG12-Biotin; Biotin-PEG12-ketone; 1,11-Dioxo-4,7,10-trioxa-2-azadodecane-12-yl 5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoate PEGylated; Biotinylated PEG Diketone Linker; Heterobifunctional PEG-Biotin with Diketone; Ketone-PEG12-Biotin; Biotin-Polyethylene Glycol-Diketone
EINECS	Contact for details

Quality Control

Our Diketone-Peg12-Biotin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional group analysis. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or cGMP-grade requirements for advanced bioconjugation applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Diketone)	≥ 90%
Functional Group Assay (Biotin)	≥ 90%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.