Description

Tamra-Peg3-Biotin is a high-purity, multifunctional chemical conjugate designed for advanced bioconjugation and labeling applications. This compound integrates the bright, photostable fluorescence of the TAMRA dye with the high-affinity binding of biotin, linked by a triethylene glycol (PEG3) spacer for enhanced solubility and reduced steric hindrance. It is an essential reagent for researchers and developers in the life sciences and diagnostic industries requiring precise, dual-mode detection and imaging. Key users include pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research laboratories focused on assay development, cellular imaging, and biomolecule tracking.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, or oligonucleotides for fluorescence microscopy (FL), flow cytometry, and immunohistochemistry (IHC).
• Streptavidin/Biotin System Enhancement: Utilizes the biotin moiety for strong, specific binding to streptavidin-coated surfaces, beads, or detection reagents, while the TAMRA fluorophore provides a red-orange emission signal (~550-580 nm).
• Diagnostic Assay Development: Critical component in the development of fluorescent immunoassays (FLIA), in-situ hybridization (FISH) probes, and other clinical diagnostic platforms.
• Cellular and Tissue Imaging: Used for labeling and tracking cellular components, receptors, or drug delivery vehicles in live-cell or fixed-cell imaging studies.
• Bioconjugation & Probe Synthesis: Serves as a versatile building block for creating custom fluorescent-biotinylated probes, sensors, and molecular tools.
• Drug Discovery Research: Applied in high-throughput screening (HTS), target identification, and pharmacokinetic studies involving fluorescence-based detection.

Basic Information

Product Name	Tamra-Peg3-Biotin
CAS No.	2279944-59-9
Molecular Formula	C52H68N8O11S
Molecular Weight	1013.22 g/mol
Synonyms	5(6)-TAMRA-PEG3-Biotin; TAMRA-PEG3-Biotin; Carboxytetramethylrhodamine-PEG3-Biotin; 6-TAMRA-PEG3-Biotin; Biotin-PEG3-5-TAMRA; Biotin-PEG3-TAMRA; Biotin-(PEG)3-TAMRA; N-(Biotinoyl)-N'-(5-carboxytetramethylrhodaminoyl)triethyleneglycol diamine
EINECS	Contact for details

Quality Control

Every batch of Tamra-Peg3-Biotin is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high purity and batch-to-batch consistency through advanced techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting identity, purity, and other critical specifications. Our quality commitment supports applications requiring regulatory compliance in research and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Red-orange to purple solid or film
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to theoretical mass
Purity (HPLC)	≥ 95%
Single Isomer Content (HPLC)	≥ 90% (5- or 6-isomer)
Extinction Coefficient (ε)	~90,000 cm-1M-1 at 555 nm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.