Description

Aminooxy-Amido-Peg4-Propargyl is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring orthogonal aminooxy and propargyl reactive groups. This compound is engineered for the site-specific conjugation of biomolecules, enabling the creation of stable, well-defined bioconjugates for advanced research and development. It is an essential tool for scientists and engineers in bioconjugation, pharmaceutical development, and diagnostic assay formulation, where precise and stable linker chemistry is paramount.

Application

• Bioconjugation and Protein/Peptide Modification: Site-specific labeling of proteins, antibodies, or peptides via oxime ligation (aminooxy) with aldehyde/ketone groups and subsequent click chemistry (propargyl) for attachment of tags, dyes, or drugs.
• ADC (Antibody-Drug Conjugate) Linker Development: Serves as a functionalized PEG spacer to connect cytotoxic payloads to monoclonal antibodies, improving solubility and pharmacokinetics.
• Surface Functionalization and Biomaterial Engineering: Modification of nanoparticles, biosensors, or hydrogel surfaces to introduce bio-orthogonal handles for further immobilization of biomolecules.
• Diagnostic Reagent Synthesis: Construction of stable immunoconjugates for ELISA, lateral flow assays, or other diagnostic platforms requiring robust linker chemistry.
• Chemical Biology and Probe Development: Creation of activity-based probes or bifunctional crosslinking agents for studying protein interactions and cellular pathways.
• Polymer and Dendrimer Synthesis: As a building block for synthesizing functionalized PEG-based polymers or dendrimers with tailored reactive sites.

Basic Information

Product Name	Aminooxy-Amido-Peg4-Propargyl
CAS No.	2253965-03-4
Molecular Formula	C20H36N2O9
Molecular Weight	448.5 g/mol
Synonyms	Propargyl-PEG4-aminooxyacetamide; Aminooxy-PEG4-Propargyl; 2-[2-[2-[2-[2-(Prop-2-yn-1-yloxy)ethoxy]ethoxy]ethoxy]ethoxy]ethyl (2-aminooxyacetyl)carbamate; O-(2-Aminooxyethyl)-O'-(2-propynyl)nonacosaethylene glycol; Heterobifunctional PEG crosslinker, Aminooxy/Propargyl; BocNH-PEG4-Propargyl (deprotected form)
EINECS	Contact for details

Quality Control

Our Aminooxy-Amido-Peg4-Propargyl is manufactured under strict quality protocols to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional group integrity. We adhere to cGMP principles where applicable to support its use in pharmaceutical development workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (NMR/Titration)	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.