Description

Biotin-PEG5-CH2CH2COOH is a high-purity, heterobifunctional polyethylene glycol (PEG) linker designed for bioconjugation. This compound is critical for creating stable, site-specific linkages between biotinylated molecules and other functional groups, such as carboxylic acids, enabling advanced research and development. It is essential for scientists and manufacturers in the pharmaceutical, diagnostic, and life sciences industries who require precise and reliable conjugation tools for drug delivery systems, assay development, and biomaterial engineering.

Application

• ADC (Antibody-Drug Conjugate) Development: Serves as a cleavable or non-cleavable linker to connect cytotoxic payloads to monoclonal antibodies, enhancing therapeutic targeting and efficacy.
• Diagnostic Assay Reagents: Used to conjugate detection enzymes (e.g., HRP, alkaline phosphatase) or fluorescent dyes to biotinylated antibodies or probes for ELISA, lateral flow, and other immunoassays.
• Biomaterial and Surface Functionalization: Facilitates the immobilization of biotinylated proteins, peptides, or nucleic acids onto carboxyl-functionalized surfaces, chips, or nanoparticles for biosensor and microarray applications.
• Drug Delivery System Fabrication: Enables the construction of sophisticated nanocarriers (e.g., liposomes, polymersomes) by linking targeting ligands (via biotin-streptavidin interaction) to the carrier matrix.
• Pull-Down and Affinity Purification: Acts as a key reagent for preparing biotin-labeled bait molecules to isolate interacting partners from complex biological samples using streptavidin-coated beads.
• Protein and Peptide Modification: Provides a method for introducing a biotin tag onto proteins or peptides via their available amine groups, expanding utility in proteomics and structural biology studies.

Basic Information

Product Name	Biotin-PEG5-CH2CH2COOH
CAS No.	2252330-85-9
Molecular Formula	C31H55N3O11S
Molecular Weight	677.85 g/mol
Synonyms	Biotin-PEG5-acid; Biotin-PEG5-COOH; Biotin-PEG5-Propionic Acid; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-(2-(3-carboxypropoxy)ethoxy)ethoxy)ethoxy)ethyl)pentanamide; Biotinamidopentyl-PEG5-propionic acid; Biotin-PEG5-C2 acid; EZ-Link Biotin-PEG5-COOH; Biotinylated PEG linker
EINECS	Contact for details

Quality Control

Our Biotin-PEG5-CH2CH2COOH is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional group analysis, to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and specification results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, Methanol, Water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.