Description

Dbco-Peg1-Acid CAS NO 2228857-38-1 is a heterobifunctional crosslinker featuring a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) and a carboxylic acid group for further conjugation via amide bond formation. This compound is essential for creating stable, bioorthogonal linkages in complex biomolecular assemblies without the need for cytotoxic copper catalysts. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, pharmaceutical development, and advanced material science for labeling, drug delivery, and surface functionalization applications.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: For site-specific labeling of proteins, antibodies, and peptides via the DBCO-azide click reaction, followed by acid-mediated coupling to amines.
• Polymer and Dendrimer Functionalization: As a key building block for modifying polymer backbones or dendrimer surfaces to introduce click-reactive handles for further assembly.
• Surface Immobilization and Biosensor Fabrication: To create functionalized surfaces on chips, beads, or nanoparticles for capturing azide-tagged biomolecules in diagnostic assays.
• Lipid and Nanoparticle Labeling: For incorporating tracking or targeting moieties onto lipid-based drug delivery systems, such as liposomes or lipid nanoparticles (LNPs).
• Chemical Biology and Proteomics: As a tool compound for studying biological processes through bioorthogonal labeling strategies in live cells or complex mixtures.
• Hydrogel and Biomaterial Crosslinking: To introduce crosslinking points in hydrogel matrices for tissue engineering and 3D cell culture applications.

Basic Information

Item	Details
Product Name	Dbco-Peg1-Acid
CAS No.	2228857-38-1
Molecular Formula	C₂₀H₁₇NO₄
Molecular Weight	335.36 g/mol
Synonyms	DBCO-PEG1-COOH; DBCO-acid; DBCO-C1-acid; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (2,5-dioxopyrrolidin-1-yl) (3-((2-(2-(2-carboxyethoxy)ethoxy)ethoxy)propyl)carbamate; Dibenzocyclooctyne-PEG1-acid; DBCO-PEG₁-acetic acid; Azadibenzocyclooctyne-PEG1-acid; ADIBO-PEG1-acid
EINECS	Contact for details

Quality Control

Our Dbco-Peg1-Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high purity and consistent performance for sensitive bioconjugation workflows. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.