Description

Fmoc-Nme-Peg4-Nhs Ester CAS NO 2228857-30-3 is a specialized heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and an Fmoc-protected amine. This compound is engineered for the controlled, site-specific conjugation of biomolecules, offering a hydrophilic PEG spacer to enhance solubility and reduce aggregation. It is an essential reagent for researchers and manufacturers in pharmaceutical development, bioconjugation, and proteomics, enabling the synthesis of peptide-drug conjugates, antibody-drug conjugates (ADCs), and functionalized biomaterials.

Application

• Synthesis of Antibody-Drug Conjugates (ADCs): Used to attach cytotoxic payloads to monoclonal antibodies via lysine residues, utilizing the hydrophilic PEG4 spacer to improve pharmacokinetics.
• Peptide and Protein Modification: Enables the site-specific labeling or functionalization of peptides and proteins with fluorophores, biotin, or other molecules for diagnostic and research applications.
• Biomaterial and Hydrogel Fabrication: Serves as a crosslinking agent to incorporate bioactive peptides into polymer matrices for tissue engineering and controlled drug release systems.
• Solid-Phase Peptide Synthesis (SPPS): Acts as a building block for introducing PEGylated linkers during automated peptide synthesis to enhance solubility and bioavailability of therapeutic peptides.
• Surface Functionalization: Used to create amine-reactive surfaces on biosensors, microarrays, and nanoparticles for immobilizing biomolecules.
• Prodrug Development: Facilitates the creation of cleavable linker systems in prodrugs, where the Fmoc group can be selectively removed to trigger drug release.

Basic Information

Product Name	Fmoc-Nme-Peg4-Nhs Ester
CAS No.	2228857-30-3
Molecular Formula	C35H46N2O12
Molecular Weight	686.75 g/mol
Synonyms	Fmoc-NH-PEG4-NHS Ester; Nα-Fmoc-Nω-maleimidotrioxatridecan-succinimidyl ester; 2,5,8,11-Tetraoxatetradecanedioic acid, 14-[[(9H-fluoren-9-ylmethoxy)carbonyl]amino]-3,6,9,12-tetraoxa-2,13-dioxo-, 1-(2,5-dioxo-1-pyrrolidinyl) ester; Fmoc-15-amino-4,7,10,13-tetraoxapentadecanoic acid NHS ester; Fmoc-N-amido-PEG4-NHS ester; Fmoc-NH-PEG4-NHS; Fmoc-PEG4-NHS Ester
EINECS	Contact for details

Quality Control

Our Fmoc-Nme-Peg4-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and structural confirmation by NMR and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation chemistry. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.