Description

Fmoc-Peg12-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and an Fmoc-protected amine. This compound is essential for creating stable, hydrophilic spacers in bioconjugation, significantly improving solubility and reducing steric hindrance in the final constructs. It is a critical reagent for researchers and manufacturers in pharmaceutical development, proteomics, and advanced materials science who require precise and reliable PEGylation.

Application

• Bioconjugation and PEGylation: Used to attach peptides, proteins, or other biomolecules to surfaces, nanoparticles, or other polymers via a long, flexible PEG spacer.
• ADC (Antibody-Drug Conjugate) Development: Serves as a key linker component to connect cytotoxic payloads to antibodies, enhancing pharmacokinetics and stability.
• Peptide Synthesis & Modification: Enables the site-specific introduction of a functionalized PEG chain during solid-phase peptide synthesis (SPPS) using the Fmoc-protecting group strategy.
• Hydrogel and Biomaterial Fabrication: Acts as a crosslinking agent to create biocompatible hydrogels with tunable properties for drug delivery and tissue engineering.
• Diagnostic Reagent Preparation: Utilized to label antibodies, enzymes, or other probes with fluorescent dyes or other detection molecules via the NHS ester group.
• Surface Functionalization: Applied to modify biosensor chips, microarray slides, or chromatography media with amine-containing ligands.

Basic Information

Product Name	Fmoc-Peg12-Nhs Ester
CAS No.	2227246-92-4
Molecular Formula	C73H116N2O30
Molecular Weight	~1477.7 g/mol
Synonyms	Fmoc-NH-PEG12-NHS Ester; Fmoc-Amino-PEG12-NHS; Nα-Fmoc-12-amino-4,7,10,13,16,19,22,25,28,31,34,37-dodecaoxatetratetracontan-43-oic acid 2,5-dioxopyrrolidin-1-yl ester; Fmoc-PEG12-NHS; 1-[(9H-Fluoren-9-ylmethoxy)carbonylamino]-3,6,9,12,15,18,21,24,27,30,33,36-dodecaoxaoctatetracontan-48-oic acid 2,5-dioxopyrrolidin-1-yl ester; Fmoc-12-amino-4,7,10,13,16,19,22,25,28,31,34,37-dodecaoxatetratetracontanoic acid NHS ester; Fmoc-N-amido-PEG12-NHS
EINECS	Contact for details

Quality Control

Our Fmoc-Peg12-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) detailing purity, moisture content, and functional group integrity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or waxy solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.