Description

Mal-PEG24-NHS Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a maleimide group on one terminus and an N-hydroxysuccinimide (NHS) ester on the other. This compound matters for its ability to create stable, site-specific conjugates between thiol-containing molecules (like cysteine residues in proteins or peptides) and primary amines, a cornerstone technique in bioconjugation and therapeutic development. It is essential for researchers and manufacturers in biopharmaceuticals, diagnostics, and advanced material science who require precise and reliable PEGylation to enhance drug solubility, stability, and pharmacokinetics.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via engineered cysteine residues.
• Protein and Peptide PEGylation: Modification of therapeutic proteins (e.g., interferons, enzymes) to reduce immunogenicity, increase circulatory half-life, and improve stability.
• Diagnostic Reagent Preparation: Conjugation of enzymes, fluorophores, or other reporter molecules to antibodies, peptides, or oligonucleotides for ELISA, flow cytometry, and imaging assays.
• Nanoparticle and Liposome Functionalization: Surface modification of drug delivery vehicles to attach targeting ligands (e.g., peptides, antibodies) for active targeting.
• Hydrogel and Biomaterial Synthesis: Crosslinking agent for creating PEG-based hydrogels with tailored mechanical properties and biofunctionalization capabilities for tissue engineering.
• Surface Plasmon Resonance (SPR) Chip Coating: Immobilization of thiolated biomolecules onto amine-functionalized sensor surfaces for interaction studies.
• Biosensor Development: Construction of stable, oriented biorecognition layers on transducer surfaces.
• Small Molecule Prodrug Synthesis: Creating cleavable linkages between amine-containing drugs and thiol-reactive targeting moieties.

Basic Information

Product Name	Mal-PEG24-NHS Ester
CAS No.	2226733-37-3
Molecular Formula	C58H104N2O28
Molecular Weight	~1301.5 g/mol
Synonyms	Maleimide-PEG24-NHS Ester; MAL-PEG24-NHS; NHS-PEG24-MAL; α-Maleimido-ω-N-hydroxysuccinimidyl ester poly(ethylene glycol) (n~24); N-(3-Maleimidopropionyl)-ω-N-hydroxysuccinimidyl poly(ethylene glycol) ether; SM(PEG)24; Succinimidyl ester of Maleimide-PEG24 acid; 3-Maleimidopropionic acid N-hydroxysuccinimide ester poly(ethylene glycol) derivative
EINECS	Contact for details

Quality Control

Our Mal-PEG24-NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for critical bioconjugation applications. Certificates of Analysis (COA) are provided, detailing key parameters such as assay (HPLC), functional group substitution ratio, and impurity profiles. We support compliance with relevant guidelines for research and pre-clinical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture condensation. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous liquid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Functional Substitution (NMR)	≥ 95%
Maleimide Content	≥ 0.95 mol/mol
NHS Ester Content	≥ 0.95 mol/mol
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.