Description

Biotin-PEG4-Picolyl Azide is a high-purity, heterobifunctional crosslinking reagent designed for bioorthogonal conjugation strategies. Its core value lies in enabling precise, site-specific labeling and immobilization of biomolecules through the combination of biotin-streptavidin affinity and copper-free click chemistry. This compound is essential for researchers and developers in pharmaceutical R&D, proteomics, and diagnostic assay development who require reliable and efficient tools for constructing complex bioconjugates.

Application

• ADC (Antibody-Drug Conjugate) Development: Site-specific conjugation of cytotoxic payloads to antibodies via bioorthogonal chemistry.
• Proteomics & Biomarker Discovery: Selective labeling and enrichment of target proteins or glycoproteins for mass spectrometry analysis.
• Diagnostic Assay Development: Immobilization of capture antibodies or antigens on streptavidin-coated surfaces for ELISA and lateral flow assays.
• Cell Surface Engineering: Labeling and tracking of cell surface receptors or other membrane proteins in live-cell imaging.
• Chemical Biology Probes: Construction of activity-based probes for studying enzyme function and metabolic pathways.
• Nanoparticle Functionalization: Conjugation of targeting ligands (e.g., antibodies, peptides) to nanoparticles for targeted drug delivery.
• DNA/RNA Sequencing Technologies: Attachment of sequencing adapters or other functional moieties to nucleic acids.
• Materials Science: Creating biofunctionalized hydrogels or polymer surfaces for tissue engineering and biosensor applications.

Basic Information

Product Name	Biotin-PEG4-Picolyl Azide
CAS No.	2222687-71-8
Molecular Formula	C₃₀H₄₇N₉O₈S
Molecular Weight	669.8 g/mol
Synonyms	Biotin-PEG4-Picolyl-N₃; Biotin-PEG4-2-Picolyl Azide; 6-((6-((2-(2-(2-(2-(2-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)amino)-6-oxohexyl)oxy)-2-(azidomethyl)pyridine; Biotin-PEG4-Alkyne (common functional analog); Biotinylated PEG4 Azide; Picolyl Azide-PEG4-Biotin; N-(Azidomethyl)pyridine-PEG4-Biotin Conjugate
EINECS	Contact for details

Quality Control

Our Biotin-PEG4-Picolyl Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.