Description

Bis-Peg21-Nhs Ester CAS NO 2221948-98-5 is a high-purity, heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester group at each terminus. This compound is engineered to facilitate the stable, covalent conjugation of biomolecules, such as proteins, peptides, and antibodies, with other functional entities or surfaces. It is an essential reagent for researchers and manufacturers in the fields of bioconjugation, diagnostics, and advanced therapeutic development, where precise and reliable crosslinking is paramount.

Application

• Bioconjugation Reagent: For the covalent attachment of proteins, peptides, or antibodies to other biomolecules, nanoparticles, or solid supports.
• Diagnostic Assay Development: Used in the preparation of enzyme-labeled antibodies or antigens for ELISA, lateral flow assays, and other immunoassay platforms.
• Drug Delivery Systems: As a linker for creating PEGylated drug conjugates, enhancing solubility, stability, and pharmacokinetic profiles of active pharmaceutical ingredients.
• Surface Functionalization: For modifying biosensor surfaces, microarray slides, or chromatography media with amine-containing ligands.
• Biomaterial Engineering: Crosslinking agent in the development of hydrogels or functionalized polymers for tissue engineering and regenerative medicine.
• Antibody-Drug Conjugate (ADC) Research: A key intermediate in the synthesis of linkers for connecting cytotoxic payloads to monoclonal antibodies.

Basic Information

Product Name	Bis-Peg21-Nhs Ester
CAS No.	2221948-98-5
Molecular Formula	C52H92N2O27
Molecular Weight	1177.3 g/mol (approx.)
Synonyms	Bis(NHS)PEG21; Bis(succinimidyl) PEG21 ester; α,ω-Bis(N-hydroxysuccinimidyl ester) poly(ethylene glycol), MW ~1000; PEG-1000 bis(NHS ester); 1,1'-[1,11-dioxo-3,6,9,12,15,18,21,24,27,30,33,36,39,42,45,48,51,54,57,60,63-henicosaoxahexahexacontane-1,65-diyl]bis[2,5-pyrrolidinedione]; NHS-PEG21-NHS; Bis-Succinimidyl PEG21
EINECS	Contact for details

Quality Control

Our Bis-Peg21-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store in a freezer.

Specification

Item	Specification
Appearance	White to off-white solid or waxy solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (NHS Ester)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.