Description

Methyltetrazine-Peg4-Nh-Boc CAS NO 2194563-84-1 is a heterobifunctional crosslinking reagent featuring a methyltetrazine click chemistry handle and a Boc-protected amine, connected by a flexible tetraethylene glycol (PEG4) spacer. This compound is engineered for the site-specific conjugation of biomolecules, enabling the construction of stable, well-defined bioconjugates for advanced research and development. It is an essential tool for scientists in pharmaceutical discovery, bioconjugate chemistry, and materials science who require precise and efficient labeling strategies.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to antibodies via bioorthogonal tetrazine ligation.
• Fluorescent Probe Synthesis: Labeling of peptides, proteins, and oligonucleotides with fluorophores for imaging and diagnostics.
• Polymer and Biomaterial Functionalization: Modification of surfaces, hydrogels, and nanoparticles with bioactive ligands.
• Chemical Biology Tool Development: Creation of activity-based probes and affinity tags for target identification and validation.
• Protein-Polymer Conjugation: PEGylation or attachment of other polymers to enhance pharmacokinetic properties of therapeutic proteins.
• Diagnostic Assay Development: Immobilization of capture antibodies or antigens on solid supports for ELISA and biosensor applications.

Basic Information

Product Name	Methyltetrazine-Peg4-Nh-Boc
CAS No.	2194563-84-1
Molecular Formula	C27H45N7O8
Molecular Weight	595.70 g/mol
Synonyms	Boc-NH-PEG4-Methyltetrazine; 1-(1,4,7,10-Tetraoxa-13-azatridecan-13-yl)-2-(4-(1-methyl-1H-1,2,4,5-tetrazin-3-yl)benzyl)malonate tert-butyl ester; Methyltetrazine-PEG4-amine-Boc; 3-(4-((2,5,8,11-Tetraoxatetradecan-14-yl)oxy)benzyl)-6-methyl-1,2,4,5-tetrazine; Boc-Amino-PEG4-Methyltetrazine; Methyl-Tetrazine-PEG4-NHBoc; Tetrazine-PEG4-NH-Boc
EINECS	Contact for details

Quality Control

Our Methyltetrazine-Peg4-Nh-Boc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and development protocols. Custom purity grades and analytical packages are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange viscous oil or solid
Identification (NMR)	Complies with structure
Identification (MS)	Complies (M+H+ or M+Na+)
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.