Description

Tco-Peg12-Nhs Ester is a heterobifunctional crosslinking reagent featuring a tetrazine cyclooctyne (TCO) group for bioorthogonal click chemistry and an amine-reactive N-hydroxysuccinimide (NHS) ester. This compound is engineered to enable efficient, rapid, and catalyst-free conjugation of biomolecules, such as antibodies or peptides, to various probes and surfaces under physiological conditions. It is an essential tool for researchers and developers in the fields of bioconjugation, targeted drug delivery, and advanced diagnostic assay development, particularly for time-sensitive labeling applications.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via bioorthogonal chemistry.
• Fluorescent Probe Labeling: Enables the fast and efficient labeling of proteins, peptides, or oligonucleotides with tetrazine-reactive fluorophores for live-cell imaging.
• Pretargeted Radioimmunotherapy: A key component in multi-step pretargeting strategies for delivering radionuclides to tumor sites with improved pharmacokinetics.
• Surface Functionalization: Used to modify nanoparticles, biosensors, or microarray slides with amine-containing ligands for enhanced targeting and detection.
• Protein-Protein Crosslinking: Facilitates the study of protein interactions and the construction of protein complexes through stable covalent linkages.
• Polymer and Biomaterial Modification: Incorporates bioactive motifs into hydrogels or polymeric drug carriers to create smart, responsive delivery systems.

Basic Information

Product Name	Tco-Peg12-Nhs Ester
CAS No.	2185016-39-9
Molecular Formula	C₄₀H₆₈N₄O₁₆
Molecular Weight	861.0 g/mol
Synonyms	trans-Cyclooctyne-PEG12-NHS Ester; TCO-PEG₁₂-NHS Ester; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-((2,5-dioxopyrrolidin-1-yl)oxy)-1-oxo-3,6,9,12,15,18,21,24,27,30,33,36-dodecaoxa-2-azahentetracontan-41-yl)carbamate; TCO-PEG12-NHS; TCO-PEG-NHS Ester (12 Unit); Tetrazine Cyclooctyne PEG NHS Ester
EINECS	Contact for details

Quality Control

Our Tco-Peg12-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive bioconjugation applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.