Description

Cy5 Azide is a functionalized near-infrared cyanine dye derivative designed for bioorthogonal click chemistry applications. This compound is essential for researchers and developers requiring high-performance labeling and conjugation in complex biological systems. It is primarily utilized in the life sciences, pharmaceutical, and diagnostic industries for advanced biomolecular tagging, imaging, and assay development.

Application

• Bioorthogonal Labeling: Conjugation with alkyne-modified biomolecules (proteins, antibodies, oligonucleotides) via copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC).
• Fluorescent Imaging: Near-infrared (NIR) fluorescent probe for in vitro and in vivo imaging applications, offering deep tissue penetration and low background autofluorescence.
• Diagnostic Assay Development: Key component in developing fluorescent immunoassays, flow cytometry panels, and fluorescence in situ hybridization (FISH) probes.
• Drug Discovery: Labeling of drug candidates or targets for tracking distribution, binding studies, and pharmacokinetic analysis.
• Materials Science: Surface functionalization and labeling of nanoparticles, polymers, and other materials for tracking and characterization.
• Proteomics & Genomics: Fluorescent tagging of proteins, peptides, or nucleic acids for gel-based analysis, microarray detection, and next-generation sequencing workflows.

Basic Information

Product Name	Cy5 Azide
CAS No.	2183440-50-6
Molecular Formula	C37H45N6O3S2+
Molecular Weight	693.92 g/mol (for cation)
Synonyms	Sulfo-Cyanine5 azide; Cyanine5 N3; Sulfo-Cy5 Azide; 1-(5-Carboxypentyl)-2-((1E,3E)-5-(1-(6-azidohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate; NIR Cy5 azide; Azide-functionalized Cy5 dye; Click chemistry Cy5 dye
EINECS	Contact for details

Quality Control

Our Cy5 Azide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and performance characteristics are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 5.0 %
Extinction Coefficient	≥ 250,000 cm-1M-1 (at λmax ~ 649 nm in aqueous buffer)
Functional Performance	Conjugation efficiency ≥ 90% (by HPLC/MS)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.