Description

Disulfo-Cy3 Amine is a water-soluble, near-infrared fluorescent dye derivative featuring a reactive primary amine group. This compound is essential for researchers and manufacturers requiring stable, bright labeling of biomolecules with minimal impact on their biological activity. It is primarily used by professionals in the life sciences, diagnostics, and pharmaceutical development sectors for applications such as fluorescence microscopy, flow cytometry, and in vivo imaging.

Application

• Bioconjugation and Probe Synthesis: Used as a reactive intermediate for covalently labeling antibodies, proteins, peptides, and oligonucleotides with a bright, sulfonated cyanine 3 (Cy3) fluorophore.
• Fluorescence Microscopy & Imaging: Enables high-contrast, photostable labeling for fixed-cell and live-cell imaging studies due to its enhanced water solubility and reduced dye aggregation.
• Flow Cytometry & Cell Analysis: Serves as a key component in developing fluorescent detection reagents for cell surface marker analysis and intracellular staining.
• In Vivo Imaging Agent Development: The disulfo groups improve hydrophilicity, making the dye conjugate suitable for preclinical research and diagnostic probe development.
• Diagnostic Assay Development: Incorporated into lateral flow assays, ELISA kits, and other bioassays as a stable fluorescent reporter molecule.
• Biomaterial and Nanoparticle Labeling: Functionalizes polymers, dendrimers, and nanoparticles for tracking and biodistribution studies in biomedical research.

Basic Information

Product Name	Disulfo-Cy3 Amine
CAS No.	2183440-43-7
Molecular Formula	C34H41N3O8S22- • 2X+ (X = counter ion)
Molecular Weight	~683.84 g/mol (Free acid form)
Synonyms	Sulfo-Cyanine3 amine; Cy3™ disulfonated amine; 2-((1E,3E)-5-((E)-2-((E)-3-(2-((2,5-Dioxopyrrolidin-1-yl)oxy)-2-oxoethyl)-2-methyl-3-(4-sulfonatobutyl)-1H-indol-1-ium-1-yl)vinyl)-3,3-dimethyl-5-sulfonato-1-(3-sulfonatopropyl)indolin-2-ylidene)ethan-1-ide amine derivative; Disulfonated Cy3 NHS ester amine derivative; Water-soluble Cy3 amine; Suflo-Cy3 amine; ICG derivative amine
EINECS	Contact for details

Quality Control

Our Disulfo-Cy3 Amine is produced under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic verification (UV-Vis, fluorescence, MS). Certificates of Analysis (COA) detailing purity, identity, and performance metrics are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue solid or lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with molecular structure
Purity (HPLC)	≥ 95% (Area percent at λabs max)
Absorption Maximum (λmax)	548 - 552 nm (in aqueous buffer)
Emission Maximum (λem)	562 - 568 nm (in aqueous buffer)
Extinction Coefficient (ε)	≥ 150,000 cm-1M-1
Solubility	Freely soluble in water, PBS, and other aqueous buffers
Water Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.