Description

Tco-Peg9-Maleimide is a heterobifunctional crosslinking reagent featuring a trans-cyclooctene (TCO) group and a maleimide moiety, connected by a hydrophilic polyethylene glycol (PEG9) spacer. This compound is specifically engineered for bioorthogonal click chemistry applications, enabling rapid, selective, and high-yield conjugation under mild physiological conditions. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and advanced drug delivery systems, where precise and stable linkage of biomolecules is critical.

Application

• Antibody-Drug Conjugate (ADC) Linker: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via the TCO-tetrazine inverse electron-demand Diels-Alder (IEDDA) reaction.
• Protein and Peptide Labeling: Enables the modification of cysteine residues on proteins or peptides with TCO handles for subsequent bioorthogonal tagging.
• Surface Functionalization: Used to introduce reactive TCO groups onto nanoparticles, biosensors, or microarray surfaces for capturing tetrazine-modified ligands.
• In Vivo Pretargeting Strategies: A key component in multi-step pretargeting approaches for radioimmunotherapy and molecular imaging.
• Polymer and Biomaterial Modification: Facilitates the crosslinking or functionalization of hydrogels and other polymeric materials for biomedical applications.
• Diagnostic Probe Development: For creating targeted imaging probes by conjugating targeting vectors (via maleimide) with reporter molecules (via TCO).

Basic Information

Product Name	Tco-Peg9-Maleimide
CAS No.	2183440-37-9
Molecular Formula	C₃₄H₅₉N₃O₁₂
Molecular Weight	701.85 g/mol
Synonyms	trans-Cyclooctene-PEG9-Maleimide; TCO-PEG9-MAL; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-oxo-2,5,8,11,14,17,20,23,26-nonaoxaoctacosan-28-yl) carbamate; TCO-PEG9-NHS Ester (precursor); TCO-PEG₉-Maleimide; 2,5-Dioxopyrrolidin-1-yl 3-((2-(2-(2-(2-(2-(2-(2-(2-(2-((((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl)methoxy)carbonyl)amino)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)carbamoyl)propanoate
EINECS	Contact for details

Quality Control

Our Tco-Peg9-Maleimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure identity, strength, and composition. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee the product meets the high standards required for research and biopharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, moderately soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.