Description

Methyltetrazine-Peg8-Acid CAS NO 2183440-33-5 is a heterobifunctional crosslinker featuring a methyltetrazine group for bioorthogonal click chemistry and a terminal carboxylic acid for convenient conjugation to amines. This compound is engineered for high reactivity and stability, enabling efficient and specific labeling of biomolecules in complex environments. It is an essential reagent for researchers and developers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and advanced materials science.

Application

• Bioconjugation and ADC Development: Site-specific conjugation of payloads (e.g., toxins, dyes, drugs) to antibodies, proteins, or peptides via the tetrazine-trans-cyclooctene (TCO) inverse electron demand Diels-Alder (IEDDA) reaction.
• Chemical Biology & Proteomics: Labeling and imaging of biomolecules in live cells or complex biological samples for target identification and pathway analysis.
• Diagnostic Reagent Synthesis: Construction of sensitive probes and contrast agents for in vitro diagnostics (IVD) and in vivo imaging applications.
• Polymer and Materials Science: Functionalization of surfaces, nanoparticles, and hydrogels to create smart materials with bio-responsive properties.
• Pre-targeting Strategies: A key component in multi-step pre-targeting approaches for radioimmunotherapy and imaging, improving target-to-background ratios.
• Linker for Solid-Phase Synthesis: The PEG8 spacer enhances solubility and reduces steric hindrance, making it suitable for use as a soluble linker in peptide and oligonucleotide synthesis.

Basic Information

Product Name	Methyltetrazine-Peg8-Acid
CAS No.	2183440-33-5
Molecular Formula	C34H60N6O13
Molecular Weight	760.87 g/mol
Synonyms	5-Methyl-1,2,4,5-tetrazine-3,6-dicarboxylate-PEG8-acid; Methyltetrazine-PEG8-COOH; m-Tetrazine-PEG8-acid; 3-(2-(2-(2-(2-(2-(2-(2-(5-Methyl-1,2,4,5-tetrazine-3,6-dicarbonyl)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)propanoic Acid; TZ-PEG8-COOH; MeTz-PEG8-Acid; Heterobifunctional Tetrazine-PEG8 Linker
EINECS	Contact for details

Quality Control

Every batch of Methyltetrazine-Peg8-Acid is manufactured under strict quality management systems and undergoes comprehensive analytical characterization to ensure identity, purity, and performance. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from HPLC, NMR, and mass spectrometry. Our quality commitment supports applications in research and pre-clinical development, adhering to the high standards required for sensitive bioconjugation work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange viscous oil or solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.