Description

N3-Peg8-Ch2Co2-Nhs is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide (N3) group and an N-hydroxysuccinimide (NHS) ester, designed for efficient bioconjugation and click chemistry applications. This compound is critical for creating stable, site-specific linkages between biomolecules, nanoparticles, and surfaces, enabling advanced research and development in life sciences. It is primarily needed by researchers and manufacturers in the fields of biopharmaceuticals, diagnostics, and materials science for applications such as antibody-drug conjugate (ADC) development, surface functionalization, and hydrogel formation.

Application

• Antibody-Drug Conjugate (ADC) Development: Facilitates the controlled attachment of cytotoxic payloads to monoclonal antibodies via the NHS ester and subsequent copper-free click chemistry with alkyne-modified molecules.
• Protein and Peptide Labeling: Enables site-specific modification of lysine residues on proteins or peptides with azide-containing probes, dyes, or other functional groups for imaging and detection.
• Surface Functionalization: Used to create biofunctional coatings on nanoparticles, chips, or beads by reacting the NHS ester with surface amines, presenting azide groups for further conjugation.
• Hydrogel and Biomaterial Fabrication: Serves as a crosslinking agent to form PEG-based hydrogels through dual-reactive click chemistry, useful for 3D cell culture and drug delivery systems.
• Diagnostic Assay Development: Employed in the construction of sensitive immunoassays by conjugating detection enzymes or fluorescent tags to antibodies or antigens.
• Polymer and Dendrimer Modification: Acts as a versatile linker to introduce azide functionality into polymer backbones or dendrimer structures for further modular assembly.

Basic Information

Product Name	N3-Peg8-Ch2Co2-Nhs
CAS No.	2182601-81-4
Molecular Formula	C33H58N4O15
Molecular Weight	750.83 g/mol
Synonyms	Azido-PEG8-NHS ester; N3-PEG8-NHS; 1-Azido-3,6,9,12,15,18,21,24-octaoxaheptacosan-27-oic acid 2,5-dioxopyrrolidin-1-yl ester; Azido-PEG8-NHS Ester; NHS-PEG8-Azide; Azido-PEG8-N-hydroxysuccinimide ester; PEG8 spacer, azide and NHS ester; Azido-OEG8-NHS ester
EINECS	Contact for details

Quality Control

Our N3-Peg8-Ch2Co2-Nhs is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and NMR for structural confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, moderately soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.